E. coli DNA polymerase I
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Last Update: 2020-10-31
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Source: Internet
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Author: User
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E. coli is
There
three main functions of DNA polymerase (E. Coli DNA polymerase): (1) 5'→3' polymerization. But instead of copying chromosomes, it repairs DNA, filling gaps in DNA or leaving gaps left by the removal of RNA quotations. (2) 3'→ 5' extase activity. Eliminates errors and nucleotides
polymerization
. (3) 5' → 3' exoceps activity. Remove the damaged DNA. It is applied in nick translation.
E. coli DNA polymerase I. Klenow fragment is a fragment of complete DNA polymerase I. that is only active in 5'→3 polymerases and 3'→5' exoceps activity, and loses 5'→3 exocetic enzyme activity. It can be used to fill the end of a single strand of DNA into a double strand. If a 32P-labeled triphosphate nucleotide is supplied, the DNA can be
with a
isotope. When a DNA fragment with a single-stranded viscous end is cut with a staggered cutting restriction enzyme, and the DNA fragments that are cut into flat-head ends are used, the single-stranded patch at the sticky end can be used to make the flat head first, and then the two DNA fragments can be connected under the action of DNA connective enzymes.is also E. coli DNA polymerase II. and E. coli DNA polymerase III. The former cannot use single-stranded DNA or poly (dA-dT) as a template. Magnesium ions and dNTP are required to exhibit enzyme activity and do not spontaneously synthesize DNA. The latter does not have → 5' and 3 extase activity and cannot use single-stranded DNA as a template.
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