Effect of hinge region in CAR T carrier on CAR T cell amplification, differentiation and lethal activity.

In recent years, chisellular antigen-treated T-cell (CAR T) therapy has shown excellent results in many clinical trials, and CAR T vectors have developed into third- and fourth-generation vectors.
the upgrading of CAR T carriers mainly focuses on the study of co-stimulating molecules, but little is known about the role of Hinge in CAR T structures.
March 13, the Li Peng Research Group of the Guangzhou Institute of Biomedicine and Health of the Chinese Academy of Sciences published its findings in the international academic journal Journal of Hematology and Oncology, entitled "Incorporation of a hinge domain improves the expansion of the chimeric antigen receptor T cells."
study group systematically studied the effects of hinge regions on CAR T cell amplification, differentiation, and lethal activity in in vitro and in vivo.
Li Peng's research team first constructed a series of CAR T cells (for CD19, Mesothelin, PSCA, MUC1, HER2) that contained and did not contain the Hinge structure, and then compared the effects of the Hinge structure on THE cell amplification and killing activity in in-body.
study found that the introduction of the hinge structure into the CAR T vector significantly promotes the amplification capacity of CAR T cells, and mainly promotes the amplification of CD4-CAR T cells, while also enhancing the migration capacity of CAR T cells.
the study further used tumor mouse models to demonstrate in vivo that the Hinge structure can effectively enhance lethal activity against Mesothelin-specific CAR T cells.
this study provides a new method for CAR T cell amplification and a new strategy for CAR T carrier design.
the research was supported by projects such as the Stem Cell Pilot of the Chinese Academy of Sciences, the National Natural Science Foundation of China and the Guangdong Provincial Fund for Distinguished Young People in Natural Science.
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