Effect of silibinin on angiogenesis

2012-11-07 classification: efficacy 0 people commented that the growth of solid tumors depends on the continuous and extensive angiogenesis, which is also the main way of tumor metastasis Inhibition of angiogenesis has become an important means of tumor treatment Yoo Hg and others treated ECV304 cells with silybin, the results showed that the growth of endothelial cells was significantly inhibited, and a large number of cells apoptosis The results showed that caspase-9 and caspase-3 were activated, PARP was cleaved and cytochrome C was released from mitochondria DNA electrophoresis showed ladder changes, Bax up-regulated and Bcl-2 down regulated, suggesting that silybin inhibited angiogenesis by inducing endothelial cell apoptosis Singh RP et al Fed with 0.05% or 0.01% silybin, found that the tumor volume and weight of prostate cancer DU145 cell xenograft mice were significantly reduced, and cell apoptosis was observed Endothelial cell specific antigen CD31 staining showed that microvessel density decreased significantly, 38% lower than that of the control group No obvious side effects of silybin were found in the experiment In addition, researchers co cultured LoVo cell line and endothelial cell ea.hy926, and then used silybin, SM and thalidomide, which were less than ic20 dose of the two cell lines, to act on the culture lines respectively It was found that the three drugs significantly inhibited the tendency of endothelial cells to LoVo cells and the formation of endothelial cells tubular Vascular endothelial growth factor (VEGF) is one of the important angiogenic regulatory factors The content of VEGF in the culture medium was detected The results showed that VEGF was significantly reduced, and Sm and silybin were more effective than thalidomide Jiang C It was also reported that CYM can reduce the growth factor of human vascular endothelial cells, and make endothelial cells apoptosis, capillary formation of endothelial cells The ability was inhibited obviously.