Effects of insulin and epinephrine on blood sugar concentration.

. The normalblood sugar is relatively constant, and the amount of 70 to 110 mg/dl on an empty stomach is necessary to maintain various physiological activities. The constant blood sugar content is regulated by various factors, the
hormone
regulation is an important part of it, insulin can reduce blood sugar, and adrenaline has the effect of raising blood sugar, the purpose of this experiment is to observe the effect of these two hormones on blood sugar content.
operation is . Take two rabbits (more than 4 hours on an empty stomach), record the weight separately, scrape the hair from the ear veins with a blade, and follow the following steps. 1. The effect of insulin on blood sugar content
(1) take a rabbit with xylene to rub the root of the rabbit's ear, to allow the blood vessels to fully expand, cut through the ear veins with a blade, so that blood drip into the anticoagulant bottle (take blood about 2mL), with the drop shake, to prevent coagulation.
(2) the amount of insulin to be injected by weight (3 to 4 units/kg body weight). Under-skin injections, record time, and keep an eye on the animal to prevent shock and rescue as soon as it occurs.
(3) after 1 hour of injection, and then the same blood draw. 2. The effect of epinephrine on blood sugar content: take another rabbit basic operation on the same, injection of epinephrine (1:1000) dose of 0.4 mg/kg body weight, because the effect of epinephrine is faster, after half an hour of injection can take blood. 3. Blood sugar quantification: Blood samples taken before and after the injection of hormones from two rabbits were measured for blood sugar.
note: . Preparation of anticoagulant bottles:
can be used in small
test tubes
or empty penicillin vials, choose one of the following anticoagulants:
1. Sodium tetamine tetetate (
EDTA
-2Na)
one of the preferred anticoagulants for blood glucose determination. Time is divided into 1% aqueous solution, anticoagulant dosing is 1 to 2 mg/mL blood, at room temperature
dry
.
2. The anticoagulant
is 2 mg/mL blood, not more than 3 mg/mL blood, and can be dried at 80 degrees C. It is easy to break down into carbonates if the temperature reaches 100 degrees C.
3. Sodium fluoride (NaF)
is used in 4 mg/mL blood, is a glycolytic inhibitor, also has anticoagulant effect, often mixed with potassium aureate, EDTA..