eLife Li Yulong's laboratory discovers a new transporter located in synaptic vesicles

In the nervous system, chemical synaptic transmission depends on specific neurotransmitters stored in synaptic vesicles and their release when nerve cells are active

Recently, the Li Yulong laboratory of Peking University published a research paper titled "Localization, proteomics, and metabolite profiling reveal a putative vesicular transporter for UDP-glucose" online in eLife magazine .

At present, it is known that the neurotransmitter transporters located in synaptic vesicles belong to the Solute Carrier ( SLC ) family, and more than half of the about 450 members of this family still lack in-depth research [2]

Figure 1 Fluorescence imaging and electron microscopy imaging show that the SLC35D3 transporter can be localized in synaptic vesicles

(A) Top: Schematic diagram of SLC transporters for screening synaptic vesicles localization by fluorescence imaging

In order to explore the possible substrates of the orphan transporter SLC35D3 , the authors used a targeted metabolic small molecule analysis method to find the metabolic small molecules that were enriched in the vesicles overexpressing SLC35D3

Figure 2 Schematic diagram of UDP-glucose transporter located in synaptic vesicles

Interestingly, previous studies on G protein-coupled receptors found that P2Y14 receptors can be activated by UDP- glucose [3] , which further suggests that UDP- glucose may act as a new type of extracellular signaling molecule

Professor Li Yulong from the School of Life Sciences of Peking University is the corresponding author of the article, and Qian Cheng , a PhD student in the PTN project, is the first author of this article

Original link: https://elifesciences.

Reference

[1] Blakely, RD, and Edwards, RH (2012).

[2] Cesar-Razquin, A.

[3] Chambers, JK, Macdonald, LE, Sarau, HM, Ames, RS, Freeman, K.