ELISA kit for detection of carp vitellogenin

Many ecotoxicological testing laboratories used in the standard test of OECD, the development of the kit for quantitative VTG of carp (Cyprinus carpio) species The kit can also be used to quantify VTG in Pimephales promelas, another widely used species of Cyprinidae The assay used to analyze plasma samples has been optimized, but it can also be used for other types of samples (e.g whole body homogenates) This analysis is based on the use of specific binding between antibodies and VTG on the sandwich method In the microtitration plate, there is a specific capture antibody coated with a VTG in advance, and the sample combined with VTG is added to the hole Unbound washing component, with a different VTG specific antibody (detection antibody) added, creates a sandwich antibody and VTG specific antibody Wash unbound test antibodies, enzyme labeled secondary antibodies, and added sandwich binding After a washing of Zui, the enzyme activity was determined by adding this to give a colored product to the substrate The activity (color intensity) of the enzyme is directly proportional to the concentration of VTG in the sample The standard of purified VTG from carp is used for calibration and determination The law is highly sensitive and powerful The standard of VTG in the working range is normal: 0.98-125 ng commodity source / ml and the coefficient of variation (CV) between batches is 5.6% and 24% in the working range There are two sizes of ELISA kits for VTG in carp, 96 and 480 holes (1 and 5 plates) The kit contains precoatet microtitration plate, VTG standard of Carassius auratus, required antibodies and reagents for running detection, and an enlightening protocol.