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Engineering Cyclic Amidases for Non-natural Amino Acid Synthesis

 Last Update: 2020-12-09
 Source: Internet
 Author: User

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keratin amino acids

amino acid derivatives

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Hydantoinases/dihydropyrimidinases are important biotechnological enzymes involved in the production of α- and β-amino acids. Their isolation from new sources with different substrate specificities, improved activity, enantioselectivity, or higher stability continues to be of great industrial interest. Here, we provide a detailed description of how to produce high quantities of the recombinant hydantoinase/dihydropyrimidinase enzyme from
Sinorhizobium meliloti
CECT4114 (SmeDhp). Several techniques are combined to obtain this goal, from cloning to activity measurement by HPLC.

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