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Enzymatic Synthesis and Characterization of Unmodified Ribozymes and Substrates

 Last Update: 2020-12-30
 Source: Internet
 Author: User

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This chapter provides the basic procedures for RNA handling, transcription reactions, and transcript purification (
1
,
2
). For further analysis, the RNAs can be end-labeled. Simple procedures generate a sequence ladder to confirm colinearity of template and transcript (
3
,
4
). This is not a “mere luxury,” since examples with significant template slippage have been reported (
5
). If a small labeled cleavage product is produced, also the exact cleavage sites can be determined (
6
).

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