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So far, no fixed method has been widely applied to each enzyme, and specific enzymes have to choose a specific fixation method according to the specific application purpose. The established fixed method can be divided into three categories: carrier binding method (including physical adsorption). Ion binding method, the legal. co-price binding method), cross-linking method and encapsulation method (including polymer encapsulation method, hydrophobic interaction, microcapsules, lipid encapsulation). carrier binding method: (1) physical adsorption method: is the earliest method used to prepare a fixed enzyme, it is sufficient to be based on physical adsorption on the surface of the body, so that the enzyme a water insoluble carrier contact and achieve the purpose of enzyme adsorption. co-price cross-linking method:co-price cross-linking method is through dual-function or multi-functionalreagent, in the enzyme molecules or between enzymes and carriers to form a co-price bond connection method. These reagents with two identical or different functional groups are called crosslinkers. Common crosslinkers are shunbutene diacid and ethylene co-polymers, dialdehyde and so on, of which dialdehyde is the most commonly used. encumeration method:A fixed method for burying enzymes in a polymer gel mesh or highly divided into a semi-permeable membrane; The encumulation method generally does not need to bind to the" >amino acid residue of the enzyme protein, less changes in the advanced structure of the enzyme, the recovery rate of the enzyme is higher, but it is only applicable to the small substrate and product of the enzyme, because only small molecules can spread into the human polymer gel mesh, and this diffusion resistance will also lead to a change in the dynamic behavior of the fixed enzyme and reduced vitality. Microcystic method is the use of various types of membranes to close the enzyme, such membranes can make low molecular products and substrates through, and enzymes and other high score in the substance can not pass. Fiber enculation is a method of burying enzymes in the micro-holes of synthetic fibers, which has the advantage of low cost and large surface area that can be used for enzyme binding. There is excellent anti microbiology and antichemical reagents performance, the most commonly used polymer is cellulose acetate. (Responsible Editor: King Kunlun of Great Han) |