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rats angiosin " ІІ 。 (Ang ІІ ) enzyme-linked immunoanalysing ( ). ELISA ")
< "center" style: "TEXT-INDENT: 28.1pt; TEXT-ALIGN: center;"> < "FONT-SIZE: 14.0pt"; ">box instruction manualreagents for research purposes only < style">Purpose: This kit is used to determine rats serum plasma, cell cleansing and related liquidsamplesangioanin ІІ ( ІІ < FONT-SIZE: 14.0pt;COLOR:black;">) < "FONT-SIZE: 12.0pt;COLOR: black;">"content.
experimental principle:
< style""> this kit applies a double"> antibody clamping method to determine the rats in the specimenangioanin < "color:black;" >ІІ " Ang ІІ" With purified ratsangiosin ІІ( Ang ІІ ) antibody package is microplate, made solid-phase antibody, into the packet of mono-resistant micro-holes in turn added angiospan style < "color;">ІІ " <"color:black;">AngangІІ ) <" "">" and HRP -labeled sheep anti-rat antibodies bind to form antibodies - ." Antigens - ase-labeled antibody complex, after thorough washing of the substrate TMB color display. TMB is transformed into blue by the catalysis of HRP enzyme, and into the final yellow with the effect of acid. The shade of color and the rats in the sample angiosin ІІ " ( ІІ )" " Measure the absorbance at <"">450nm wavelengths" ( OD " value), and calculate rats in the sample using a standard curve < "color: black;"> angioanin ІІ " <"color"black;">AngІІ < "color: black;" >) concentration.
" kit composition :
kit composition | <td style="BORDER-RIGHT: windowtext 1.0pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: windowtext 1.0pt solid; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0.0cm; BORDER-LEFT: medium none; WIDTH: 126.0pt; PADDING-TOP: 0.