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    Home > Biochemistry News > Biotechnology News > Rat angiotensin ІІ (Ang ІІ) enzyme-linked immunoassay (ELISA)

    Rat angiotensin ІІ (Ang ІІ) enzyme-linked immunoassay (ELISA)

    • Last Update: 2020-12-16
    • Source: Internet
    • Author: User
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    rat angiosin " ІІ (Ang ІІ ) enzyme-linked immunoanalysing ( ). ELISA ")

    < "center" style: "TEXT-INDENT: 28.1pt; TEXT-ALIGN: center;"> < "FONT-SIZE: 14.0pt"; ">box instruction manual

    reagents for research purposes only <; style>Purpose: This kit is used to determine rats serum plasma, cell serum and related liquidsamplesangioanin ІІ ( ІІ < FONT-SIZE: 14.0pt;COLOR:black;">) < "FONT-SIZE: 12.0pt;COLOR: black;">"content.

    experimental principle:

    < style"">" this kit using dualantibody clamping method to determine the specimen ratsangioanin < "color: black;" >ІІ"black" Ang ІІ" With purified ratsangiosin ІІ ( Ang ІІ ) antibody package is microplate, made solid-phase antibody, into the packet of mono-resistant micro-holes in turn added angiosin ІІ ( AngІІ )" < "">" and HRP labeled sheep anti-rat antibodies, forming antibodies - Antigens - ase-labeled antibody complex, after thorough washing of the substrate TMB color display. TMB is transformed into blue by the catalysis of HRP enzyme, and into the final yellow with the effect of acid. The shade of color and the rats in the sample angioanin ІІ ( "ІІ " " Measure the absorbance at <"">450nm wavelengths" ( OD " value), and calculate rats in the sample using a standard curve < "color: black;"> angioanin ІІ " <"color"black;">AngІІ < "color: black;" >) concentration.

    " :

    kit composition

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