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rat alcoholic fatty liver (AFL) enzyme-linked immunoanalysis
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<" "MsoNormal" style "TEXT-INDENT: 24.0pt;" >reagents for research purposes only < 0pt;COLOR: black;">" Purpose: This kit is used to determine ratserumThe content of alcoholic fatty liver (SFA) in plasma, cellularandliquid samples.experimental principle:
< ""> Kits are used with a doubleantibody sandwich method to determine the level of alcoholic fatty liver disease ( AFL "). Antibody packs with purified rat alcoholic fatty liver (") wereMicroplate plate , made into solid-phase antibodies, into the packet of mono-resistant micro-holes in turn to add alcoholic fatty liver ( AFL ),< with the">HRPlabeled alcoholic fatty liver ("< >"The antibody ">" binds to form the antibody- Antigens - ase-labeled antibody complex, after thorough washing plus substrate TMB color display. TMB is transformed into blue by the catalysis of HRP enzyme, and into the final yellow with the effect of acid. The shade of color and the alcoholic fatty liver ( AFL " is positively related. Measure the absorbent at 450nm wavelength" ( OD> value), the concentration of alcoholic fatty liver disease in rats ( AFL ") was calculated using a standard curve.
kit consists :
kit consists of | 48 。 Hole configuration | < b style "" > " < 96" "> style" |
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1 。 part
