< "FONT-WEIGHT: bold; FONT-SIZE: 14.0pt; COLOR: rgb (0,0,0); "> Rats free fatty acids ( FFA) ase-immune < "FONT-WEIGHT: bold; FONT-SIZE: 14.0pt;"> analysis
reagents < "FONT-WEIGHT: bold; FONT-SIZE: 14.0pt;" > box instruction manual
reagents are for research purposes only Purpose: This kit is used to determine rats serum plasma and related liquid samples free fatty acids (FFA) content.
Experimental Principles < "FONT-WEIGHT; FONT-SIZE: FONT-SIZE: FONT-SIZE 10.5pt;">:
" This kit is "FONT-SIZE: 10.5pt;" > dual antibody clamping measurement < < FONT-SIZE: 10.5pt; > "FONT-SIZE: 10.5pt;" in > Rats < "FONT-SIZE: 10.5pt; COLOR: rgb (0,0,0); > free fatty acids (FFA) < "FONT-SIZE: 10.5pt;" > level. With purified free fatty acids (FFA) antibodys pack is microplate to make solid phase , the package is " "Font-SIZE <: 10.5pt; COLOR: rgb (0,0,0); > free fatty acids (FFA) are added to micro-holes ," then HRP labeled free fatty acids (). FFA) antibody binds to form antibodies "Times New Roman" >- < a href" > < "Times New Roman" >- enzyme Standard antibody complex , after thorough washing < FONT-SIZE: 10.5pt; "> plus substrate <": "Times New Roman" > TMB color display. TMB at HRP enzyme catalytic conversion to blue, and under the effect of acid into the final yellow. The shade of color and the free fatty acids (FFA) is positively related. Use an enzyme labeler to measure absorbentity at 450 nm wavelength ( OD through the standard curve calculated sample rats in free fatty acids (FFA content.
The kit consists of :
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