Enzyme-linked immunoassay of rat advanced glycation end products (AGEs)

Saccharide-based end product (AGEs < "FONT-SIZE: 14.0pt;" >-linked immunoanalytogenic

"box instruction manual

Reage >nts are for research purposes only <purpose: this kit Used to determine the content of , late stagestyle" in plasma andrelated liquid samples in rats

experimental principle:

" Double clamping method to determine the late rats in the specimenglycosylation end product(AGEs)level. With purified rat lateglycosylation end product(AGEs)antibody package ismicroplate to make solid-phase antibodies, to the packet of monoantigenic micro-holes In turn, add the late span style"color: black;" > (AGEs),"andP."saccharide-based final product (AGEs)antibody binds to form antibodies -><"> a href""">antigen - ase-labeled antibody complex, after thorough washing of the substrateTMBcolor display color. TMBis transformed into blue by the catalysis ofHRPenzyme, and into the final yellow with the effect of acid. The depth of color and the late span style(AGEs)" is positively related. Measure the absorbent at450nmwavelengths" ( ODvalue"), The late ratglycosylation end product "color: black"; "> AGEs" (AGEs < "" > concentration was calculated using a standard curve.

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