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Estrogen (E) enzyme-linked immunology analysis (ELISA) in mice

 Last Update: 2020-10-26
 Source: Internet
 Author: User

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mouse estrogen hormone(E) enzyme Immunoanalysis (ELISA)

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reagents for research purposes only: This kit is used to determine miceserum. , plasma and related liquid samples contained estrogen (E).

experimental principle:

kit uses a dual antibody sandwich method to determine estrogen (E) levels in mice in the specimen. With purified mice estrogen (E) antibody packagemicroplate to make a solid-phase antibody, into the packet of single-resistant micro-holes added estradiol (E), and then In combination with the HLP-labeled esodiol (E) antibody, the antibody is formed -" - an enzyme-labeled antibody complex, which is thoroughly washed and colored with the substrate TMB.

< p style is "text-align:left;" >TMB is catalyzed by HRP enzymes into blue and, under the effect of acid, into the final yellow. The shade of color is positively related to estrogen (E) in the sample. The absorbance (OD value) is measured at a wavelength of 450 nm using an enzymatic marker, and the level of estrogen (E) in mice in the sample is calculated using a standard curve.

kit composition:

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