Experiment 58 Determination of antholin content

The determination of anthratin contentprinciple anthratin is one of the widely distributed pigments in plants, belongs to flavonoids
compounds
, flavonoids in plant growth play a regulatory role, has been paid attention to.antholin in different pH conditions, showing different colors, in acidity is red, its color depth and anthotin content proportional, with color method can be measured, the method is simple and easy.instrument721 type classification photon thermometer thermometer
balance
scissorsbeothmeasuring barrel 0.1 mol/L HCloperating stepsThe material is called 1g, cut into 23mm pieces, placed in the beaker, add 0.1mol/L Hcl 10ml (depending on the anthratin content and increase or decrease HCl dosing), cup mouth with weighing paper cover tight, to waterproof evaporation. Place in a temperature box at 32 degrees C and soak for at least 4 hours. Then
filter
, take the filter with
hydrometer
at the wavelength of 530nm to read the absorbent, with 0.1 mol/L Hcl as a blank control, with a light diameter of 1cm color cup. Anthratin concentrationscalled 1 unit when the absorbent is 0.1 to compare the relative content of anthratin. The measured absorbent is multiplied by 10 to represent the relative concentration unit of antholin.。