Experiment 8 Determination of pore density and area of plant leaves

Experiment 8 The determination of the density and area of the pores of plant leavesPrinciple The steaming of the pores occupies an important position, while the number of pores on the leaf surface and the size of the pores are closely related to the intensity of the steaming of the pores, so understanding the distribution and area of the pores on the leaf surface is of great significance for understanding the steaming effect of plants.the number of pores on theunit area can be counted using
microscope
and then the diameter of the field of view is measured by an orthometer to find the field of view area, thus calculating the number of pores on the per unit leaf surface. The measurement of the area of the pores is calculated by drawing on millimeter grid paper with the help of a microscope depictor.instrumentmicroscope object measure microscopeplotter (or microscope imager) slide, cover slopemm square paper operating steps 1. Determine the number and density of poresput the surface plate on fresh leaves (or below) under a microscope to calculate the number of pores in the field of view (with a low-fold mirror or a high-fold mirror, determined by the number of pores on the skin), move the production, and count 56 times at different parts of the skin to average them. The diameter of the field of view is then measured by an objector, the radius r is known, the field of view area is calculated according to the formula S-r2 (S is the field of view area), and the density of the pores can be calculated by using the average/field of view area of the pores in the field of view, expressed as "the number of pores/mm2".2. To measure the area of the pores is to measure the total area of the pores on the leaf surface, as long as the average area of a pore can be measured, because the density of the pores has been sequestred according to the method.Secure the millimeter square paper to the tabletop on the right side of the microscope with a pushpin, adjust the reflector on the profiler to make a 45-degree tilt, and then adjust the brightness of the light, so that the image of the pore and the millimeter square paper in the microscope field of view, with a pencil on the square paper to draw a number of pores, so that the enlarged pores in an area equal to 20mm2, the actual area of the air hole must also know the microscope. To this end, the objector scale is placed under the microscope, such as the former method to draw a number of micrometers scale on the grid line, to determine that the enlarged micrometer each scale is equivalent to several mm, because the actual length of each small scale of the objector micrometer is known to be 10 m, so the microscope magnification can be obtained. For example, if each scale of the micrometer is equal to 5mm on millimeter grid paper, the magnification of the microscope is 500.the magnification of the length is determined, it is also required to calculate the magnification of the area, from which the actual area can be known to be 100 m2, after enlarged to 25mm2. The actual area of the pores can be reached by proportional method.100∶25=X∶30 。