echemi logo
Product
  • Product
  • Supplier
  • Inquiry
    Home > Biochemistry News > Biotechnology News > Expression of Foreign Genes in Mammalian Cells

    Expression of Foreign Genes in Mammalian Cells

    • Last Update: 2020-12-07
    • Source: Internet
    • Author: User
    Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

    Many laboratories are currently interested in cloning cellular genes that code for polypeptides that may have academic or therapeutic use. Once the intact gene is isolated, usually in the form of a c
    DNA
    clone, it has to be engineered into an expression cassette that is capable of functioning when introduced into a particular host cell. The choice of using a prokaryotic (e.g., bacterial), lower eukaryotic (e.g., yeast), or higher eukaryotic (e.g., mammalian) expression system is frequently dictated by the nature of the polypeptide to be expressed. It is becoming increasingly evident that for the majority of cloned eukaryotic genes there can be considerable problems in achieving reliable expression of biologically active products in bacterial or yeast systems. Although the product may be expressed in high amounts in these systems, often it is not folded correctly and is therefore biologically inactive or is produced in an insoluble, denatured form within occlusion bodies.
    This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

    Contact Us

    The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

    Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.