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    Home > Biochemistry News > Biotechnology News > Extraction, separation and identification of seven-leaf glycosides and seven-leaf esters in Qin skin.

    Extraction, separation and identification of seven-leaf glycosides and seven-leaf esters in Qin skin.

    • Last Update: 2020-10-17
    • Source: Internet
    • Author: User
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    the bark of fraxinus Chinensis Poxb, or F. rhynchophylla Hance, or F. bungeana DC, a white wax tree, tastes bitter and chills. It has the effect of clear heat, dryness and dampness. The main treatment of warm dysentery, red tumors and other diseases.qin skin contains a variety of endoester components and saponins, tannins and so on, of which there are mainly seven-leaf glycosides, seven-leaf esters, qin-pinine and qin-skinin and so on. There are many antibacterial anti-inflammatory physiological activity, seven-leaf esters on bacterial dysentery, acute enteritis has a better therapeutic effect, both the role of heat regeneration, the role of poison pay is small, little bitter taste. Suitable for children.structure and properties of the main components in Qin skin
    1. esculin, also known as thyroid bark glycoside: white powdered crystallization, mp205 to 206 degrees C. Soluble in hot water (1:15), soluble in ethanol (1:24), slightly soluble in cold water (1:610), insoluble in ethyl acetate, insoluble in ether, chloroform. Hydrolysed in thin acids. There is blue fluorescence in the aqueous solution.2. esculetin: yellow needle crystallization, mp276 degrees C. Soluble in boiling ethanol and sodium hydroxide solution, soluble in ethyl acetate, slightly soluble in boiling water, insoluble in ether, chloroform.3. fraxin: mp205 degrees C.4. fraxetin: mp227 to 228 degrees C.experimental principle
    Helioside and seven-leaf esters can be dissolved in boiling ethanol, which can be extracted by boiling ethanol and separated by different solubility in ethyl acetate.experimental method
    (i) extraction: take 150g of Qin skin crude powder in the Soshi extractor, add 400 ml of ethanol reflow 10-12 hours, get ethanol extract, reduce pressure recovery solvent to dip paste, that is, a total
    extract
    .
    (ii) separation: add 40 ml of water to the above-mentioned
    heating
    soluble. Moved in the distriging
    fleet
    , to the same volume chloroform extraction twice, chloroform extracted water layer steamed off the residual chloroform and then added ethyl acetate extraction secondary, combined ethyl acetate liquid, waterless sodium sulfate dehydration, pressure relief recovery solvent to dry, residue dissolved in warm methanol, concentrated to the appropriate amount, placed in the crystal, there is yellow needle crystallization. The crystallization is filtered out. Methanol, water repeatedly recrystration, that is, seven-leaf esters.concentrate the extracted layer of ethyl acetate to the appropriate amount and place the crystal, i.e. the micro-yellow crystal is extracted. The crystallization is filtered out. With methanol, water is repeatedly recrystineded, i.e. seven-leaf glycosides.(iii) Identification:
    1. Chemical detection: take seven-leaf glycoside, seven-leaf esters each slightly placed
    test tube
    , add ethanol 1 ml dissolved. Add 1% FeCl3 solution 2-3 drops, dark green, and then add 3 drops of ammonia water, add water 6 ml, daylight observation dark red.
    2. Thin layer identification:
    adsorbent: silicone G
    samples: seven-leaf glycoside, seven-leaf nesteride
    sysm standard
    and homemade seven-leaf glycoside, seven-leaf nesteride alcohol solution.
    expander: methanol-ethyl acetate-toluene (1:4:5)
    color: 1) UV254 lamp observation, seven-leaf glycoside gray fluorescence, seven-leaf nesteride gray-brown.2) the nitrobenzene spray was colored with heavy nitride, and the seven-leaf glycoside and seven-leaf nesteride were agate.
    results: seven-leaf glycoside RF=0.04, seven-leaf ester Rf=0.28
    .
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