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Fabrication of Dynamic Self-Assembled Monolayers for Cell Migration and Adhesion Studies

 Last Update: 2020-12-15
 Source: Internet
 Author: User

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How cells interact with the extracellular matrix (ECM) is important for a number of fundamental �processes in cell biology. However, the ECM is highly complex and in order to simplify the matrix for cell biological studies, it has been modeled with self-assembled monolayers (SAMs) of alkanethiolates on gold substrates. In this chapter, we outline procedures to create dynamic surfaces by functionalizing SAMs. SAMs based on quinone, oxyamine, and alcohol-terminated thiols were used to immobilize cell adhesive peptides with spatial control. Cells were seeded to these surfaces to provide cell co-culture �patterns suitable for biological studies.

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