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    Home > Biochemistry News > Microbiology News > Fake monocytobacteria agar base culture/CN agar

    Fake monocytobacteria agar base culture/CN agar

    • Last Update: 2021-01-24
    • Source: Internet
    • Author: User
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    culture characteristics
    < "top" width"142" >
    copper-green fake monocytobacteria
    < td valign" "top" width "142" >
    copper-green fake monocytobacteria
    < td valign""top" width"142" >
    produces yellow-green pigment
    < "top" width"142" >
    E. coli E. coli
    storage: stored in light, shady < the bottle cap immediately > thethe drying area of the battery. The storage period is three years.
    specification: 250g


    ).
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    < div style:" text-align: left;"> use:
    for the selective separation of copper-green false monocytobacteria andculture . (GB/T 8538-2008)
    principle:
    gelatin and tryprotein provide nitrogen sources; Glycerin provides a source of carbon; potassium sulfate and magnesium chloride promote the production of chlorpyric pigment; > agar is a coagulant for">ficum"; and Gelants-negative bacillus, which inhibits non-fake monocytobacteria.



    " 1, said to take this product 52.6g, add distilled water or deionized water 1 L, and add 10mL glyceryl (029080), stirring heating boiled to complete dissolution, divided into triangular bottles, 121 degrees C autocultension 15min.

    2, every 100mL when the culture base is cooled to 45 to 50 degrees C The media is added to the CN agar matchingreagents (SR0230) 1 (1.5 mg oxalic acid), mixed and poured flat Plate, media thickness of at least 5mm;


    3, water sample to be measured " filtered Attach the membrane to the prepared CN agar plate, tile and avoid holding bubbles between the membrane and the culture, and culture 40h to 48d at 36c±1C.


    <"text-align:left;">4, observation.
    "quality control:
    the following quality-controlled strains were cultured at 36 degrees C±1 degrees C after vaccination± The results of 8h are as follows:
    "
    bacteria number
    growth status
    ATCC9027
    good
    produces yellow-green pigment
    ATCC27853
    good
    ATCC25922
    suppressed>
    -
    Staphylococcus a
    ATCC25923
    suppressed>
    -