Fang Xianyang and Chen Chunlai's research group from the School of Life Sciences collaborated on the molecular mechanism of the conformational dynamics and functional regulation of flavivirus xrRNA

RNA has a variety of important physiological functions and can play a role in processes such as genetic coding, gene expression, gene regulation, and enzyme catalysis

Flaviviruses including dengue virus, Zika virus and West Nile virus can cause infection in the population and cause a series of potentially serious diseases

On November 5, 2021, Fang Xianyang, School of Life Sciences, Tsinghua University, and Chen Chunlai’s research group jointly published an online publication titled "Pseudoknot Length Regulation of Flavivirus xrRNA Folding, Conformational Dynamics and Antibiotics" in Nature Communications.

smFRET technology is an important method to study the conformational dynamics of biological macromolecules.

Figure 1.

Next, the researchers integrated small-angle X-ray scattering (SAXS), smFRET, and anti-Xrn1 digestion kinetics experimental technology to study the folding of 11 xrRNAs of different lengths (2-7 bp) of PK2 from the flavivirus family in solution , Conformation dynamics and anti-Xrn1 enzyme cleavage activity (Figure 1d-f)

Figure 2.

Researcher Fang Xianyang and Researcher Chen Chunlai of School of Life Sciences of Tsinghua University are the co-corresponding authors of this paper

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