Fat 酶 Immydation

: I fixed the lipase in the large hole adsorption resin D3520 and
dry
, want to test the enzyme activity of the lipase. However, after the resin is dried and then put into the lipase reaction system, the resin particles are floating on top of it without mixing. Could you tell me how to measure enzyme activity in this case? (Sodium hydroxide titration method to measure enzyme activity)
, even if I test enzymes live, how to recover the resin? Directly filter the reaction
,
, alcohol washing? Please give advice to your comrades, thank you! !
: I have not done fixed, is not you drying the large hole resin too much? How do you control the conditions? You can use the plate of triglycerides to take a cursive look at whether your fixed enzyme still has enzyme activity ah? Can I immerse it directly with buffer before the reaction?
also want to ask you a question, you said: "Before I react, let the enzyme and emulsion and buffer mixed evenly, do not shake." "Do you compare shaking with not shaking?" I think if you do not shake the enzyme and emulsion contact is not enough will detect the enzyme activity will be low? Because I usually look at the fixed temperature of about 200 rpm to react. Please give advice.
: I just think a certain amount of water is necessary for the maintenance of the structure or activity of lipase, so you can't do too much to fix it to the resin. I'm not sure exactly, you look for the literature. Can't you re-pin it? Did you buy the lipase or did you make it yourself? My research topic is lipase-related, currently not fixed, may be used later, later we can learn from each other.
: I am doing fixation, resin with alcohol treatment, wash clean with water will not float on the water.
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