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Background: Hashimoto's thyroiditis (HT) is a type of thyroid myeloperoxidase antibody (Thyroid peroxidase antibody, TPOAb), Thyroid globulin antibody (TgAb) increase and thyroid lymphocyte infiltration as the characteristics Of common autoimmune diseases
Myeloperoxidase thyroid thyroglobulin antibodies increased lymphocytic infiltration of the thyroid, and a common autoimmune disease characterized by a variety of autoimmune diseases
Methods: This study comprehensively used animal models, vitreous cell culture and differentiation, mRNA sequencing, 16S rRNA sequencing and other methods to systematically explore the therapeutic effects and mechanisms of metformin on HT
Results: We found that metformin does have a therapeutic effect on HT mice, mainly by reducing TgAb and lymphocyte infiltration in thyroid tissue
TgAb thyroid tissue and reduce lymphocyte infiltration suppressing HT mouse Th17 cell number and function of macrophages and M1 polarization can be suppressed in vitro T H17 differentiation and function of regulating the immune pathway Metformin may also be altered intestinal flora
Figure 1 (A) The process of making animal model experiments .
Figure 1 (A) The process of making animal model experiments .
(c) Comparison of plasma and spleen TGAb expression indexes
Figure 2 (A) Flow cytometry diagram, (B) Th1 cell ratio, (C) Th2 cell ratio, (D) Th17 cell ratio
Figure 2 (A) Flow cytometry diagram, (B) Th1 cell ratio, (C) Th2 cell ratio, (D) Th17 cell ratio
Figure 3 (A) IL-17 immunohistochemistry and immunofluorescence thyroid tissue, (A) IL-17 immunohistochemistry, (b) IL-17 immunofluorescence
Figure 3 (A) IL-17 immunohistochemistry and immunofluorescence thyroid tissue, (A) IL-17 immunohistochemistry, (b) IL-17 immunofluorescence
(a) Immunohistochemical score of IL-17 in thyroid tissue, (b) Immunofluorescence score of IL-17 in thyroid tissue, (c) ELISA to detect the expression level of IL-17 in plasma
Figure 4 Metformin affects the differentiation of naive T cells into Th17 cells
Figure 4 Metformin affects the differentiation of naive T cells into Th17 cells
(C) The differentiation ratio of Th17 cells under different metformin concentrations
Figure 5 Flow cytometry and statistics of mouse spleen neutrophils and macrophages: (A) flow cytometry chart, (B) neutrophil ratio, (C) M1 macrophage ratio, (D ) The proportion of M2 macrophages, (E) the proportion of M1-M2 macrophages
Figure 5 Flow cytometry and statistics of mouse spleen neutrophils and macrophages: (A) flow cytometry chart, (B) neutrophil ratio, (C) M1 macrophage ratio, (D ) The proportion of M2 macrophages, (E) the proportion of M1-M2 macrophages
.
Figure 6 16s-RNA sequencing to detect differences in intestinal flora
.
(A) Differences in observed species, chao index, ace index, Shannon diversity, Simpson diversity and Good coverage between groups.
(B) Distance weighted UniFrac,
.
(A) Differences in observed species, chao index, ace index, Shannon diversity, Simpson diversity and Good coverage between groups.
(B) Distance-weighted UniFrac, Figure 6 16s-RNA sequencing to detect differences in intestinal flora
.
(A) Differences in observed species, chao index, ace index, Shannon diversity, Simpson diversity and Good coverage between groups.
(B) Distance weighted UniFrac,
(C) Venn diagram of plants between groups, (D) species analysis at different levels, (a) phylum classification level, (b) class classification level, (c) order classification level, (d) family classification level, (e) ) Belongs to classification level, (f) species classification level
.
.
(C) Venn diagram of plants between groups, (D) species analysis at different levels, (a) phylum classification level, (b) class classification level, (c) order classification level, (d) family classification level, (e) ) Belongs to classification level, (f) species classification level
.
Conclusion: These experimental results provide a preliminary theoretical basis for the clinical application of metformin in the treatment of HT
.
Original source
Jia X, Zhai T, Qu C,et al, Metformin Reverses Hashimoto's Thyroiditis by Regulating Key Immune Events.
Front Cell Dev Biol 2021;9
Front Cell Dev Biol 2021;9 Metformin Reverses Hashimoto's Thyroiditis by Regulating Key Immune Events.
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