FICOLL centrifuges.

: FICOLL after centrifugal interface is not clear what is the general reason?
: There are many reasons:
1, FICOLL does not have room temperature balance;
2, blood dilution is not fully mixed;
3, when the blood is not layered clearly;
4, blood anticoagulant is not sufficient, cell block, it is recommended to take good blood after room temperature for half an hour to 1 hour period from time to time mixed;
5, individual differences, the same experimental conditions, different sources of blood may vary;
6, centrifugal speed is too small to have been completely stratified, or during centrifuge speed instability;
7, speed reduction too fast, speed reduction time should be more than 5min.
: 1, the proportion should be appropriate: diluted blood: lymphocyte separation fluid is 1:1.
2, blood tiled to lymphocyte separation fluid is clearly layered?
3,
centrifuges can not
brake suddenly, should let the speed slowly down.
.