Fluorescent Lipids as Probes for Sphingosine Kinase Activity by Capillary Electrophoresis
Last Update: 2021-01-03
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Capillary electrophoresis (CE) is one among a number of highly sensitive chemical separation techniques used to characterizesingle or a small number of cells and to develop assays of enzymatic activity. Other commonly used techniques include massspectrometry and electrochemistry; however, CE using laser-induced fluorescence detection (LIF) is the most sensitive of thesetechniques. In CE-LIF, fluorescently labeled proteins or lipids are normally separated based on their size to charge ratioin the interior of a small capillary filled with an electrolyte upon the application of an electric field. In this chapter,we describe the application of CE-LIF for the determination of the bioactivity of fluorescently lipids and sphingosine kinaseactivity.
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