For the first time, the process of human embryos sleeping in bed has been parsed

, Aug. 22 (Reporter Zhang Jiaxing) A fertilized egg grows into a baby, needs to be in bed on the 7th day of development to survive, during which the human embryonic cells can not be obtained, difficult to resolve. On the 22nd, nature magazine published the latest breakthrough in the joint team research between Tang Fufu of Peking University and Qiao Jie of the Three Hospitals of North Medicine, who demonstrated the "black box" by using in-body simulation of human bed-building strategy and high-precision single-cell sequencing technology.
" clinically, bed failure is one of the most important factors leading to early miscarriage. Qiao Jie, author of the paper,
of the Chinese Academy of Engineering and
third hospital of Peking University, said that human embryos in the early post-bed stage after natural conception could not be obtained before, and that the use of rodent embryos or monkey embryos and other model biological substitution studies could not accurately reflect the real situation of human molecular regulation and other laws.
, the team used fertilized eggs after artificial insemination to simulate bed growth in human embryos. Using high-precision single-cell multi-group sequencing technology, the team reconstructed the dynamic change process of transcription and DNA methylation groups drawn at single-cell resolution for the first time, recreated the process of human embryo beding, and mapped the gene expression control network and DNA methylation dynamic "panoramic map", said Tang Fufu, author of the paper's communication and professor at Peking University's Frontier Innovation Center for Life
and Biomedical Sciences.
" embryo initiates the preparation of the mother-to-child connection at this particular stage. Zhou Fan, a postdoctoral student at Peking University
who is one of the authors of the paper, said, for example, that the upper embryo layer exhibits erythromoric transformation (erythrocytes can differentiate into different organs, such as the brain, heart, etc.), and that nourishing the outer embryo layer gradually begins to express specific hormone-related genes. All kinds of cell linees have unique characteristic genes, and the process of bed-eating crab monkey embryos presents a certain difference.
study also found that the level of DNA methylation modification of different cell types during embryo beding showed a unique increase. "Embryonic cells may work with DNA methylation to build regulatory systems." The timing of methylation of different genes may also be involved in specifically regulating the transcription of key genes, which together determine the fate of cells, Zhou said.