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Synthetic biology technology, as a new biological technology in the 21st century, promotes the development of life science and even the whole field of natural science.
the human co-existence of the virus provides a powerful tool for revealing the nature and function of the virus, as well as the genetic transformation of the virus.
past, the exploration of virus synthesis has focused on RNA viruses, and the largest known RNA virus genome is only 30 kb.
, no more than 6 kb of DNA viruses have been reported to have been successfully synthesized.
is a large class of double-stranded DNA viruses (genome size 80-180 kb), which has important application value in biopesticides, ethyroid expression systems and other fields.
Recently, Hu Zhihong, a researcher at the Wuhan Institute of Virus Research of the Chinese Academy of Sciences, jointly used THE and yeast transformation-related homogen recombination (Transformation Associated Recombination, TAR) technology to synthesize the entire genome of ACMNPV, a rod virus model, for the first time, and successfully rescued an infectious synthetic virus by trans-infecting cells.
researchers first used PCR amplification to cover up to 45 fragments of the ACMNPV gene, each about 3kb, with overlapping sequences greater than 60 bp between adjacent fragments.
using TAR technology, three recombinations were performed in yeast cells, resulting in 9 to 15 kb fragments, 3 to 45 kb fragments, and whole genome (145,299 bp).
454 sequencing of the synthesized viral genome and successfully obtained the infectious synthetic virus AcMNPV-WIV-Syn1 (Figure A) by transflying insect cells.
such as electroscopy, step-by-step growth curve and biometrics show that synthetic viruses have similar biological characteristics to parent viruses (Figure B).
the establishment of this technology, not only provides a powerful tool for the basic research of rod virus, but also can be used to improve the expression system and insecticidal performance of rod virus.
this study is an important breakthrough in the field of large DNA virus research, the reviewer considered "this is a highly large paper that serve as a landmark in synthetic biology".
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