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Formononetin
Formononetin
[Synonym] formononetin , thorns formononetin , biogenic rather Chan B, Michel brain, daidzein 4'-methyl ether
【Structural formula】
[Molecular formula and molecular weight] C 16 H 12 O 4 ; 268.
[Source] Leguminous plant Ononis spinosa
【Properties】White fine needle-like crystals
This product is soluble in methanol , ethanol and acetone
Melting point: 255~256℃
【Purity Check】
TLC
1.
Developing solvent chloroform - methanol (30:1)
Check under UV lamp (254nm)
2.
Developing agent: ethyl acetate - butanone (3:1)
Check under UV lamp (254nm)
High performance liquid chromatography
Chromatographic column Agilent C 18 , 5um (4.
The mobile phase of methanol -1% acetic acid (60:40), 1.
Detection 250nm
【Structural identification】302,249
3132, 1639, 1610, 1568, 1514, 1452, 1385, 887, 808
ESI-MS m/z: 269 [M+H] +
1 H-NMR (DMSO) δ: 10.
13 C-NMR (DMSO) δ: 153.
【Storage】Dry and airtight