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    Formononetin

    • Last Update: 2022-05-28
    • Source: Internet
    • Author: User
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    Formononetin

    Formononetin

    [Synonym] formononetin , thorns formononetin , biogenic rather Chan B, Michel brain, daidzein 4'-methyl ether

    【Structural formula】


    [Molecular formula and molecular weight] C 16 H 12 O 4 ; 268.


    [Source] Leguminous plant Ononis spinosa


    【Properties】White fine needle-like crystals


    This product is soluble in methanol , ethanol and acetone


    Melting point: 255~256℃


    【Purity Check】

    TLC

    1.


    Developing solvent chloroform - methanol (30:1)

    Check under UV lamp (254nm)

    2.


    Developing agent: ethyl acetate - butanone (3:1)

    Check under UV lamp (254nm)

    High performance liquid chromatography

    Chromatographic column Agilent C 18 , 5um (4.


    The mobile phase of methanol -1% acetic acid (60:40), 1.


    Detection 250nm

    【Structural identification】302,249


    3132, 1639, 1610, 1568, 1514, 1452, 1385, 887, 808


    ESI-MS m/z: 269 [M+H] +


    1 H-NMR (DMSO) δ: 10.


    13 C-NMR (DMSO) δ: 153.


    【Storage】Dry and airtight


     

     

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