Formulation and procedure of four yeast cultures.

In this paper, the preparation methods and principles of four yeast cultures are introduced, including malt juice culture, potato glucose culture, bean bud juice glucose culture and Tsa's culture.
are as follows: First, the basic principles of malt juice media and potato glucose media are widely used to culture yeast and mold.
glucose cultures can sometimes also be used to culture line bacteria.
glucose culture of soybean sprout juice is also an excellent culture for the cultivation of yeast and mold.
is mainly used to culture mold observation morphology.
malt oil culture is a natural culture, potato glucose culture and bean sprout glucose culturek are both semi-synthetic culture, while Tsa's culture is synthetic culture.
pH present in the medium formulation refers to the pH that the medium naturally presents without acid and alkali regulation.
, experimental materials (1) pharmaceutical grapes, sucrose, NaN03, K2HP04, KCl, MgSO4.7H2O, FeS04, agar.
(2) instrument balance, high-pressure steam sterilization pot.
(3) glassware pipetting tube, test tube, conical bottle, beaker, cylinder, petri dish, glass funnel, etc.
(4) other items of medicine spoon, pH test paper, weighing paper, marker pen, cotton, gauze, wire rope, plastic test tube cover, veggie paper, newspapers, fresh malt juice, soybean sprouts, potatoes and so on.
, experimental content (i) malt juice culture base preparation 1. Culture base composition: fresh malt juice is generally 10-15 bolin.
2. Preparation method (1) wash barley or wheat with water, soak 6-12h with water, place in a cool place of 15 degrees C germination, cover the gauze once a day, until the malt elongates to twice the grain, let it stop germination, drying or drying, grinding into malt powder, storage reserve.
(2) Take a malt powder with four servings of water and keep it warm in a 65C water bath for 3-4h, so that it can be sugared on its own until it is fully glycedated (check the method is to take 0.5 ml of glycation liquid, plus 2 drops of iodine, if no blue appears, that is to say, complete glycation).
(3) the saccharin is filtered with 4-6 layers of gauze, if the filter is still cloudy, can be clarified with egg whites (with an egg clear, add water 20 ml, smooth to raw foam, pour into the glycerizing liquid, stir and boil, and then filter).
(4) the concentration of sugar in the glycation liquid is detected with a Pomei hydrometer, and the water of the filter is diluted to 10-15 bolin, and pH to 6.4 is adjusted.
if there is a local brewery, it can be used with fresh malt juice without fermentation or hops, diluted with water to 10-15 boreen.
(5) when paired with a solid malt medium, add 2% agar, heat and melt, replenish the water loss.
(6) sub-packing, stuffing, packing.
(7) high-pressure steam sterilization 100Pa sterilization 20min.
(ii) Potato glucose culture base preparation 1. culture base ingredients: potato 20g, glucose 2g, agar 1.5-2g, water 100ml, natural pH2. Preparation method (1) preparation 20% potato dipping peeled potato 200g, cut into small pieces, water 1000ml.
soak lh at 80 degrees C, filter with gauze, and then replenish the water loss to the desired volume.
100Pa sterilization 20min.
is 20% potato soaked in juice and stored in reserve.
(2) preparation, add 2g glucose per 100ml potato dip, heat and boil, add 2g agar, continue to heat and melt and fill with water loss.
(3) sub-packed, stuffed, wrapped.
(4) high-pressure steam sterilization 100Pa sterilization 20min.
(iii) 1. Culture base composition: soybean sprouts 10g, glucose 5g, agar 1.5-2g, water 100ml, natural pH2. The preparation method (1) is called fresh soybean sprouts 10g, placed in a beacon, then added 100ml water, a small fire boiling 30min, filtered with gauze, to make up for the loss of water, that is, to make 10% bean sprout juice.
(2) preparation, add 5g glucose per 100ml of bean sprout juice, bring to the boil and add 2g agar, continue to heat and melt to make up for the loss of water.
(3) sub-packed, stuffed, wrapped.
(4) high-pressure steam sterilization 100Pa sterilization 20min.
(iv) Czapck media preparation 1.media composition: sucrose 3g, NaN030.3g, K2HP040.1g, KCl0.05g, MgSO4.7H2O0.05g, FeS040.001g, agar 1.5-2g, distilled water 100ml, natural pH2. preparation method (1) weighing and melting amount of water required to take about 2/3 added to the beantil, respectively, called sucrose, NaNO3, K2HP04, KCl, MgSO4.
add water one by one to dissolve.
1 0.1% FeS04 solution per 100 ml of culture.
(2) after the drug is all dissolved, pour the solution into the cylinder and add water to the desired volume.
(3) add agar to the required amount of agar, heat and melt, to fill the water loss.
(4) sub-packing, stuffing, packing.
(5) high-pressure steam sterilization 100Pa sterilization 20min.
Note: The preparation of these four cultures do not need to use acid or alkali to regulate pH, sterilization 20 minutes is enough, too long easy to destroy the culture base ingredients, too short easy to cause pollution.
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