From digestive health and immunomodation to the intestinal-X axis, the "Top Ten Hot Spots in Probiotic Science Research" has been released.

In recent years, scholars at home and abroad have carried out a lot of scientific experiments on the resources, efficacy, mechanism and safety of probiotics. The rise and development of new disciplines and technologies, such as systems biology, synthetic biology and multi-group science, provides new ideas and opportunities for the development of probiotic science and technology and industry. With the emergence of many new academic views and achievements in the field of probiotic research.
In 2021-2035, the national medium- and long-term science and technology development plan and the preparation of the "14th Five-Year Plan", in order to focus on the current research hot spot of probiotic science, the probiotic research field urgently needs to crack the key common problems, the Chinese Food Science and Technology Association probiotic branch organized more than 20 authoritative experts in the field of probiotics According to the search of more than 15,000 relevant scientific and technological documents, and in conjunction with the analysis of 720 probiotic industry questionnaires collected by the branch this year from 149 units, the formation of "top ten hot spots of probiotic scientific research" and industry development recommendations were first published online on August 19 in the China Food Journal. At the 15th International Symposium on Probiotics and Health, hosted by China Food Science and Technology, "Top Ten Hot Spots in Probiotic Science Research" was officially released.1: Probiotics and Digestive Health
The human digestive tract is distributed with about 10 trillion microorganisms, and the micro-ecological balance of the digestive tract affects the health of the body. Probiotics can prevent and treat oral diseases, gastritis, stomach ulcers, irritability syndrome and inflammatory bowel disease by regulating the micro-ecological balance of the digestive tract, inhibiting the growth and adhesion of pathogenic bacteria, and reducing inflammatory responses. However, the mechanism, clinical effect and population differences of different probiotic strains need to be confirmed by evidence-based medicine. The "anaerobic intestinal chip" and the collection of capsules by gut microorganisms can provide a new method for studying the mechanism of probiotics in the intestines.Hotspot 2: Probiotics and Immunomodation
Immunity is the cornerstone of human health, and once it is dysfunctional, the body may develop health problems such as allergies, tumors, or viral infections. The intestines are known as the body's largest "immune organ" and an important place for probiotics to function. Studies have shown that probiotics can regulate the body's immune function by enhancing the function of the intestinal mucosa barrier, inhibiting the growth and adhesion of pathogenic bacteria, regulating immune cell activity and promoting the production of immune factors. However, the immunomodating effect of probiotics has strain specificity, and the screening of probiotic strains of specific antigens, the efficacy and mechanism of immunomoditation need to be studied in depth. Studying the regulation mechanism of probiotics to the immune function of the body through immuno histology, establishing the molecular library related to immunity, and analyzing the functional components and targets of the action will provide new ideas for revealing the role of probiotics in the immune regulation of the body.3: Probiotics and Metabolic Syndrome
In recent years, the incidence of metabolic syndrome characterized by metabolic disorders such as type 2 diabetes, hyperlipidemia and hypertension has been increasing, which has seriously affected public health. Intestinal microbiome disorder is one of the factors leading to metabolic syndrome, and probiotics represented by Lactobacillus and B ambiguity can regulate the micro-ecological balance of the intestine and regulate the body's metabolism to reshape the intestinal environment, thereby alleviating the occurrence and development of metabolic syndrome. At present, most studies focus on the effects of probiotics in the prevention and treatment of metabolic diseases, but the mechanism of the role of probiotics is not discussed. The main reasons are due to the complexity of the eeriology of various types of metabolic diseases, differences in the probiotic effects of different probiotic strains, and different mechanisms for probiotics to relieve metabolic syndrome.
therefore, in the future, we still need to carry out personalized probiotic screening and clinical verification for all kinds of metabolic syndromes, and further explore the role of probiotics to regulate metabolic syndrome and its mechanisms.4: Probiotics and intestinal-X-axis
90% of serotonin is produced in the intestines. Intestinal microbes regulate host emotions and behaviors by affecting the intestinal-brain axis through pathes involving nerves, immune systems, and endocrinology. In addition, enterococcus has a complex interoperability with organs and tissues such as the lungs, liver, kidneys, muscles and bones (the "intestinal-X axis"). The mutual functioning of the intestinal-X axis can be used as a new target for drug development, and probiotics can regulate body tissues, organ function and behavior through such channels as intestinal nerve, immune system and intestinal endocrine signals, which can be beneficial to host health.
although probiotics have become an effective means of regulating intestinal micro-ecological and other organ functions, their biological mechanisms are still unclear. Therefore, future research should focus on the use of multi-histology techniques to highlight the specific "contribution" of probiotics, taking into account strain specificity and the use of dosages, in order to more in-depth exploration of its mechanism of action.5: Accurate probiotic screening and functional prediction
Probiotic efficacy evaluation should focus on the level of strains, which has become a wide consensus in the scientific and technological community. Probiotic efficacy is influenced by its habitat source, host diet, age, gender and other factors, the precise screening of probiotics, need to pay attention to personalized differences. Traditional fermented foods and the intestines of healthy populations in different regions of China contain a large number of probiotic strains, which is a valuable microbial resource for screening specific functional probiotics.
adopts a step-up strain screening strategy based on physiology, genotype and target, combined with multi-group technology, multi-scale establishment of probiotic rapid and accurate screening methods and functional prediction technology, the development of personalized, functional probiotic strains for different populations, to give safe and efficient individual probiotic intervention, balance the human micro-ecological, effective prevention of disease occurrence and development, and maintain the health of the body.6: Probiotics and dietary interoperability
interoperability between diet and intestinal microbiome has become a new target for human health regulation. There are differences in the effects of different dietary patterns and food types on probiotics. Supplemental probiotics are an important means of regulating intestinal microbiomes. Probiotics can transform nutrients into new substances that are easier to absorb and more active, and dietary nutrients can promote the growth of specific probiotics. The compound consumption of the two can promote the growth of probiotics and dietary metabolism, better play health benefits.
Functional dietary supplements, represented by dietary fiber, drug-eating plant extracts, etc., have a regulatory effect on the growth and function of probiotics, regulate microbiome disorders, protect the intestinal barrier, relieve inflammation and metabolic syndrome, etc. Human genotypes and intestinal microbiomes jointly determine ideogens, and the mechanism of "food-probiotic-metabolism" and human body mutual mechanism needs further study.Hotspot 7: Probiotics and Probiotics and Regenerations / Class
After-life / Class Biogenesus are metabolites or bacterial components that can still function after inactivation treatment, with health functions such as regulating the body's immunity, protecting the intestinal barrier and regulating the intestinal microbiome. The research shows that the post-biological/class bio-yuan has stronger tolerance and stability to the physiological environment and production and processing conditions of the gastrointestinal tract, and has a good application prospect.
, limited by research techniques, most studies focus only on proteins, polysaccharides and other active substances, and do not carry out higher-resolution identification of effect substances. The separation identification and functional evaluation of probiotic metabolites are the key to the marketization of probiotics/classes. At the same time, the quality-effect relationship between the functioning of the post-biological/class meta-bions and the metabolic dynamics in the intestines also need further study.8: Probiotics and Emerging Technologies
With the continuous development of basic disciplines, more emerging technologies have been introduced into probiotic research. Whole genome sequencing, single-cell sequencing, macrogenomics, transcriptional histology, proteomics and metabolomics can reveal in depth the genetic information, evolutionary relationships, physiological characteristics, metabolic networks, functional mechanisms and their industrial application potential;
application, more emerging technologies are used in probiotic burial, drying, delivery, testing and so on. Such as the use of new wall materials to achieve layer-by-layer packaging of strains and targeted intestinal delivery, flow-based cytoscular technology to achieve rapid real-time detection of live bacteria. In the future, more cutting-edge technologies will be applied to the probiotic industry, further enhancing the industry's scientific and technological competitiveness.Hotspot 9: New Mechanisms for New Generation Probiotics and Probiotics
A new generation of probiotics is based on the development of specific symbic bacteria in the human gut, which play an important role in the balance of intestinal bacteria and host physiological function. Strains such as Akkermansia muciniphila have the potential to improve the intestinal mucosa barrier and metabolic diseases, and are candidates for a new generation of probiotics, and clinical studies on strains accumulate basic data for their drug applications. In the future, functional and safety assessments of new probiotics will be required, and applied technical research and regulatory construction will be carried out to promote the application of a new generation of probiotics. With the further research on the relationship between intestinal microbiome and host health, the target of intestinal microbiome closely related to host physiological function is gradually clarified. The effect of probiotics on the metabolic network of intestinal microbiome has become a new hot spot in the study of probiotic mechanism by regulating the target of intestinal molecular molecules to realize the intervention of probiotics to host physiology and disease. Hotspot 10: Probiotics and Edible Safety
Probiotics generally originate from traditional fermented foods or healthy human intestines, and there is no obvious risk to the host in safety evaluation or functional evaluation. The strains in China's List of Bacteria That Can Be Used in Food can be used directly in ordinary foods. At present, the widely used probiotics are Lactobacillus and B ambiobacteria, with a long history of consumption, clear sources, high safety. However, probiotic intervention is not recommended for immunodeficiency and critically ill patients. System safety evaluation is needed for new probiotic strains and a new generation of probiotics to clarify the safety of the strains. At the same time, specific safety assessment for different strains, specific populations or individuals is the future direction of probiotic food safety evaluation.
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