Gastroenterology: Vitolic acid and lymphocytoxsignalation can promote differentiation of human intestinal M cells
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Last Update: 2020-06-22
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Source: Internet
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Author: User
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Background and objective: Intestinal microfolding (M) cells are unique subtypes of intestinal epithelial cells in the Peyer spot that regulate mucous membrane immunity and serve as the first line of defense for in-cavity antigen sampling and ingestionBut current technology is not effective in growing human M-cells in cell culture, which hinders research by the intestinal immune systemTherefore, the purpose of this study is to identify the signaling pathways needed for human M-cell differentiation and to establish a robust culture system using human intestinal-like substancesmethod: The researchers analyzed transcription data from mice Peyer's Patches to identify cell populations adjacent to M cells and used a human intestinal-like system to determine the cytokines needed to induce M-cell differentiationFinally, a transcription group, immunofluoresista, scanning electron microscope and transcell swallowing experiments were carried out to verify the development requirements of the metatype and functional human M cellsresults: This study found that a combination of retinoid acid and lymphocytoxin induces glycoprotein 2-positive human M-cell differentiation, which lacks a top microfluorous structureAfter rotavirus infection, the expression of congenital immune-related genes in M cells was associated with the lack of viral antigensHuman M cells developing in the intestinal-like system internalize and transport enteroviruses, such as rotaviruses and exoroviruses, through the intestinal epithelial barrier in the intestinal sampleconclusion: This study identified the signaling pathways needed for intestinal M cell differentiation and used this information to create a powerful culture method for developing human M cells with viral internalization and transport capabilities
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