Gel lithography (gel draination analysis, molecular sieve layering, gel filtration, gel osmosis).
Last Update: 2020-11-03
Search more information of high quality chemicals, good prices and reliable suppliers, visit
Gel platoonography is also known as gel exhaust chromatography,
sieve chromatography, gel
, gel filter permeation chromatography, etc. It is a liquid phase
method for separating the components in the sample in molecular size order with the porous
as the fixed phase. In 1959, Porath and Flodin first used a porous polymer-cross-linked glucosin gel as a column filler to separate samples of different molecular weights in the aqueous solution, called gel filtration. In 1964, Moore developed a cross-linked polystyrene gel with different apertures capable of separating
solvents, called gel osmosis (gel layering with flow phases for organic solvents is commonly referred to as gel osmosis). Subsequently, this technology has been continuously improved and developed, is widely used in
, polymer chemistry and many other fields.
gel lithography is a commonly used separation method in biochemistry, it has the advantages of simple equipment, easy operation, high sample recovery rate, good experimental repeatability, especially does not change the biological activity of samples, so it is widely used in
, polysaccharides and other biological
separation purification, but also used in protein molecular weight determination, desalination, sample concentration and so on.
principle of gel
gel is to separate and purification according to the physical properties of molecular size. The layering process is shown in Figure 2-23. The fixed phase of gel layering is an inert beaded gel particle, which has a three-dimensional mesh structure inside, forming many holes. When samples containing components of different molecular sizes enter the gel
, each component spreads into the hole of the fixed phase, and the degree of diffusion of the components depends on the size of the hole and the size of the component molecule. Molecules with larger aperture than the hole can not spread to the inside of the hole, completely blocked outside the hole, only in the space outside the gel particles with the flow phase downward flow, they go through a short process, flow speed, so first flow out; Long, slow flow, so the final outflow, and the molecular size between the two molecules in the flow of partial penetration, the degree of penetration depends on the size of their molecules, so they flow out of the time between the two, the larger the components of the molecules flow out first, the smaller the components flow out later. Thus, after the sample has been analyzed by gel, the components flow out in order of molecules from large to small, thus achieving the purpose of separation.
7.2.3 Basic concepts of gel lithography
(1) outer water volume, inner water volume, substitline volume, column bed volume, extrusion volume
as shown in Fig. 2-24.
This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only.
This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of
the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed
description of the concern or complaint, to email@example.com
. A staff member will contact you within 5 working days. Once verified, infringing content
will be removed immediately.