Gene Activation by T-DNA Tagging
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Last Update: 2021-03-13
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Source: Internet
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Author: User
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Agrobacterium
-mediated transformation of plants has not only become a useful tool for the introduction of chimeric genes into plants, but it has also recently been utilized in gene tagging (for reviews,
see
refs.
1
–
3
). T-
DNA
tagging has proven a powerful genetic approach for the isolation of novel plant genes. Compared with other approaches to gene isolation (for a review,
see
ref.
4
), mutagenesis by insertion of defined DNA sequences allows the rapid cloning and characterization of the mutated locus. Furthermore, T-DNA is an ideal gene tag, as it has the potential to insert preferentially into potentially transcribed regions of the genome (
5
).
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