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    Home > Biochemistry News > Plant Extracts News > Genetic Transformation of the Model Green Alga Chlamydomonas reinhardtii

    Genetic Transformation of the Model Green Alga Chlamydomonas reinhardtii

    • Last Update: 2021-01-15
    • Source: Internet
    • Author: User
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    Over the past three decades, the single-celled green alga
    Chlamydomonas reinhardtii
    has become an invaluable model organism in plant biology and an attractive production host in biotechnology. The genetic transformation of
    Chlamydomonas
    is relatively simple and efficient, but achieving high expression levels of foreign genes has remained challenging. Here, we provide working protocols for algal cultivation and transformation as well as for selection and analysis of transgenic algal clones. We focus on two commonly used transformation methods for
    Chlamydomonas
    : glass bead-assisted transformation and particle gun-mediated (biolistic) transformation. In addition, we describe available tools for promoting efficient transgene expression and highlight important considerations for designing transformation vectors.
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