echemi logo
Product
  • Product
  • Supplier
  • Inquiry
    Home > Biochemistry News > Biotechnology News > Genomic DNA extraction method.

    Genomic DNA extraction method.

    • Last Update: 2020-10-21
    • Source: Internet
    • Author: User
    Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

    1, wall cells digested with trypsin, centrifugal collection.
    2, cells re-suspended in the cold PBS rinsing once, centrifugal collection.
    3, repeat 2.
    4, add 5ml
    . DNA
    a buffer (10m mol/L Tris-cl, 0.1 mol/L
    EDTA
    ,0.5%
    SDS
    ), mixed well.
    5, add 25ul
    protease
    K, so that the final concentration of 100ug/ml, mixed well, 50 degrees C water bath 3h.
    6, with the same volume of phenol pumping once, 2500r/min centrifugation to collect water phase. The water phase is collected at 2500r/min centrifugation with an equal volume (phenol, chloroform, isosterol) mixture.
    is used in the same volume of chloroform, isosterol pumped once.
    7, add the same volume of 5mol/L LiCL, mix well, ice bath, 10min.
    8, 2500r/min, centrifugal 10min. Turned into a
    centrifugal tube
    tube. Add isopropyl alcohol of equal volume. Room temperature for 10 minutes.
    9, 2500r/min, centrifuged 10min. Discard the Qing. Add 0.1x volume 3mol/L sodium acetate (PH5.2) and 2x volume-20C pre-cooled waterless ethanol. -20 degrees C for 20 minutes.
    10, 12000r/min, centrifuged at room temperature for 5 minutes. Discard the Qing. Dissolve the DNA in an appropriate amount of TE.
    .
    This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

    Related Articles

    Contact Us

    The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

    Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.