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Ginsenoside Ro
Ginsenoside Ro
【Synonyms】Chikusetsusaponin Ⅳ c
【Structural formula】
[Molecular formula and molecular weight] C 48 H 76 O 19;956.
[Source] The dry root of Achyranthes bidentata Bl
【Properties】White powder
This product is easily soluble in methanol and ethanol
Melting point: 239~241℃
【Purity Check】
TLC
1.
Developing solvent chloroform - methanol - water - formic acid (7:3:0.
Check 5% vanillin concentrated sulfuric acid solution, heated at 105℃ until the spots appear clear, check under sunlight
2.
Developing solvent chloroform - methanol - water (13:7:2) the lower layer liquid
Inspection of 10% sulfuric acid ethanol solution, heated at 105°C until the spots appear clear, inspect under sunlight
High performance liquid chromatography
Chromatographic column Agilent SB C 18 , 10um (4.
Mobile phase acetonitrile-ammonium bicarbonate buffer (0.
Detection wavelength 203nm
【Structural identification】203
3406(-OH), 2945(C-H), 1728(C=O), 1610(C=C), 1077(C-O)
ESI-MS m/z: 979[M+Na] + , 955[M—H] - , 793[M—gle] - , 775[M—glc—H 2 O] - , 749[M―glc—CO 2 ] -
1 H-NMR (C 5 D 5 N) δ: 0.
13 C-NMR (C 5 D 5 N) δ: 38.
【Storage】Dry and airtight
.