Glucose oxidase method to determine blood sugar content.
Last Update: 2020-10-20
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"Purposes and Requirements"
learn and master the method of
oxidase method to determine blood sugar content.
see Trinder. P. Ann. Clin. Biochem., 1969, 6:24-27.
quinoa has the maximum light absorption peak in the range of 480-550nm, and the amount of amine is directly related to the amount of glucose, the amount of niacin can be calculated by using color method to determine the amount of nicotinamide.
Reagent Stability: Original reagents are stored in 2 to 8 degrees C, valid for 15 months. After mixing, 2 to 8 degrees C can be stabilized for 1 month, room temperature can be stabilized for 3 days.
samples: fresh insoluble
. Test tube
and test tube rack, straw, pipe, piped gun,
water bath, 722 type
wavelength: 505nm (480 to 550nm) Temperature: 37
C; Mix 10mlR1 with 90mlR2, both working fluids. Table operation below:
2. Mix evenly, 37 degrees C insulation 10 to 15 minutes (avoid direct sunlight), with blank tube zero, respectively, read A standard and A sample.
note is not
. 1. The glucose concentration in the sample exceeds 22.2 mmol/L (400 mg/dl), the sample volume can be halved to 5ul, the standard amount remains unchanged, retested, the result multiplied by 2.
2. Reagent R1 is a liquid enzyme, pay attention to antifreeze
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