Glycyrrhetinic acid

Glycyrrhetinic acid

Clycyrrhetinic Acid

【Structural formula】

[Molecular formula and molecular weight] C ₃₀ H ₄₆ O ₄ ; 470.

[Source] The dried roots and rhizomes of the legume Glycyrrhiza uralensis Fisch

【Properties】White needle crystals

This product is easily soluble in methanol

Melting point: 282～284℃

【Purity Check】

TLC

1.

Developing solvent petroleum ether (30～60℃)-toluene- ethyl acetate - glacial acetic acid (10:20:7:0.

Check under UV light (365nm)

2.

Eluent petroleum ether (30 ~ 60 ℃) - chloroform - acetic acid (5 = 5: 1)

Check 10% sulfuric acid ethanol solution, heat at 105℃ until the spots are clear, check under sunlight and ultraviolet light (365nm)

Chromatographic column HilQ-sil C ₁₈ column, 5um (4.

Mobile phase acetonitrile-0.

Detection wavelength 254nm

Differential calorimetry

Start temperature 50℃, end temperature 300℃, heating rate 5℃/min

【Structural identification】204,249

3440, 1720, 1700, 1620, 1460, 1380, 1210, 1150

EI-MS m/z: 470[M] ⁺ , 303, 262, 257, 216, 175, 135

¹ H-NMR (CDCl ₃ ) δ: 0.

¹³ C-NMR (CDCl) δ: 37.
8(Cl), 27.
4(C-2), 78.
9(C-3), 39.
2(C-4), 55.
1(C-5), 18.
8(C-6), 32.
9 (C-7), 43.
3(C-8), 61.
9(C-9), 37.
2(C-10), 200.
2(C-11), 128.
7(C-12), 169.
1(C-13), 41.
1( C-14), 28.
2(C-15), 23.
4(C-16), 45.
5(C-17), 43.
8(C-18), 39.
3(C-19), 48.
4(C-20), 31.
1(C -21), 31.
9(C-22), 28.
6(C-23), 16.
4(C-24), 15.
6(C-25), 17.
6(C-26), 26.
6(C-27), 26.
6(C- 28), 28.
4 (C-29), 180.
4 (C-30)
.

【Storage】Dry and airtight
.

Related link: Naringin