Guiding Principles of DNA Barcode Molecular Identification Method for Chinese Medicinal Materials (1)

The DNA barcode molecular identification method is a molecular biology technique that uses a recognized, relatively short DNA sequence in the genome to identify species, and is an effective supplement to traditional morphological identification methods

DNA barcode molecular identification of Chinese medicinal materials usually uses ribosomal DNA second internal transcribed spacer (ITS2) as the main barcode sequence to identify Chinese medicinal materials.

1.

The instruments used are electronic balance, centrifuge, polymerase chain reaction instrument, electrophoresis instrument and sequencer

The sequencer for DNA sequence determination is a precision instrument for determining the sequence or size of DNA fragments in DNA fragments, as well as for quantification, with automatic computer control such as automatic glue filling, automatic sampling, automatic data collection and analysis

2.

This method mainly includes sample processing, DNA extraction, PCR amplification of DNA barcode sequence, electrophoresis detection and sequence determination, sequence splicing and result determination.

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Due to the wide variety of plant Chinese medicinal materials, the extraction method can be improved according to the specific conditions of the identified Chinese medicinal materials

(1) Roots, rhizomes, stems, woods, and skins: usually roots and rhizome tissues contain high levels of polyphenols and polysaccharides.

(2) Leaves, flowers, and whole plants: The DNA of these medicinal materials can generally be successfully extracted using the kit method.

(3) Fruits and seeds: Fruits and seeds of Chinese medicinal materials are rich in oils, which are easily oxidized during grinding and stick to the wall of the mortar, resulting in large losses.

(4) Animal medicinal materials: muscle animal medicinal materials such as sea dragons, snakes, geckos, etc.