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    Home > Biochemistry News > Biotechnology News > He dyeing reagent preparation is commonly used.

    He dyeing reagent preparation is commonly used.

    • Last Update: 2020-10-21
    • Source: Internet
    • Author: User
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    I. Sumu (1) Harris Sumicin:Formula: Sumicin 1 g waterless ethanol 10 ml distilled water 200 ml potassium alum 20 g HgO 0.5 gDissolve Sumulin in aqueous ethanol, spare. Put the alum into distilled water,
    heat
    dissolve, then add the spare sumuline, bring to the boil for 2 minutes, first add a small amount of mercury oxide, prevent the oxidation process of sumutin spillage, the stick stirred, and then, while stirring to add mercury oxide. Once added, immediately move to ice water, accelerate its cooling, and after a night of
    the
    . Add ice acetic acid at 5% before use. If the time after the match is longer, the amount of ice acetic acid can be appropriately added a little more. The amount of ice acetic acid can directly affect the coloring ability and clarity of sumitrin.
    add less, will cause nuclear slurry co-dyeing, the background is not clean; This liquid can be placed from March to about half a year, depending on the temperature. The use period depends on the amount of
    slices
    stained: the amount of 100 tablets per day, can be used for about half a month. In fact, if it doesn't work, it's okay not to add mercury oxide. To be naturally oxidized by sumuline. (Room temperature is different, oxidation time is also different, generally takes about three months, with the pre-filter added ice acetic acid.) This method takes a long time (2) Gill improved sumin liquid sum 2 g waterless alcohol 250 ml aluminum sulfate 17.6 g distilled water 750 ml iodized acid Sodium 0.2 g ice acetic acid 20 mlFirst the sumu essence is dissolved in aqueous alcohol, aluminum sulfate is dissolved in distilled water, then mixed with two liquids to add sodium iodate, and finally ice acetic acid. This liquid is semi-oxidized sumitin liquid, will not produce precipitation, less oxidation film. Is the formula for sexual dyeing, do not differentiate, dyeing time in 4-10 minutes or so, the effect is very good. Ideal for dyeing machines.II, Yihong(1) Yihong (water solubility)formula: Yihong (water solubility) 2.5-5 g distilled water 500 mlwater-soluble Yihong dissolved in distilled water, and then added 10 ml of hydrochloric acid, fully stirred, left overnight after filtration. The filtered precipitation is rinsed twice with distilled water and then filtered, and the sediment is placed in a temperature tank together with
    filtration paper

    driding
    , plus 95% alcohol 1000 ml, with saturated liquid. Dilute 1:1 to 2 times with 95% alcohol before use and add 1 to 2% ice acetic acid. This method has the characteristics of fast dyeing time, bright color, not easy to fade, long service life and so on.(2) Ihong (alcohol solubility)formula: iridescent (alcohol solubility) 2.5-5g 75% alcohol 1000 ml plus a few drops of ice acetic acid to translucent., fixed liquid(1)12% neutral Formarin (pH7.0) formaldehyde 120 ml NaH2PO4-H2O 4 g Na2HPO4 13 g distilled water 880 ml(2)12% acidic Formarin (PH 4.5) formaldehyde 120 ml water 830 ml ice acetic acid 50 ml(3)12% Formarin formaldehyde 120 ml water 880 ml (4) Bouin fixed liquid saturated bitter acid 75 ml Formaldehyde 25 ml ice acetic acid 5 ml 4, hydrochloric acid alcohol differentiation solution
    strong hydrochloric acid 0.5 to 1 ml 75% alcohol 99 ml
    .
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