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    Home > Active Ingredient News > Digestive System Information > Helicobacter pylori urea breath test, how to choose carbon 13 and carbon 14?

    Helicobacter pylori urea breath test, how to choose carbon 13 and carbon 14?

    • Last Update: 2022-05-15
    • Source: Internet
    • Author: User
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    Helicobacter pylori , referred to as Hp , Helicobacter pylori, habitually known as Helicobacter pylori , H.
    , can actively invade mucosal epithelial cells
    .

    Helicobacter pylori , referred to as Hp , Helicobacter pylori, habitually known as Helicobacter pylori , H.
    , can actively invade mucosal epithelial cells
    .

    Since 1983 , scientists have successfully isolated Helicobacter pylori from gastric mucosa biopsies of patients with chronic active gastritis for the first time, and it is currently the only bacteria that can survive in the strong acid environment of the stomach
    .

    Since 1983 , scientists have successfully isolated Helicobacter pylori from gastric mucosa biopsies of patients with chronic active gastritis for the first time, and it is currently the only bacteria that can survive in the strong acid environment of the stomach
    .

    Many studies have shown that the specific manifestations of complications such as gastritis, ulcers, and gastric cancer caused by Helicobacter pylori infection can also cause duodenal ulcers, gastrointestinal polyps, and colorectal cancer , resulting in constipation, diarrhea, and weight loss .

    According to the 15th edition of the latest carcinogen report released by the U.
    S.
    Department of Health and Human Services , Helicobacter pylori is listed as a clear carcinogen
    .

    Many studies have shown that the specific manifestations of complications such as gastritis, ulcers, and gastric cancer caused by Helicobacter pylori infection can also cause duodenal ulcers, gastrointestinal polyps, and colorectal cancer , resulting in constipation, diarrhea, and weight loss .

    Helicobacter pylori is listed as a definite carcinogen in the 15th edition of the latest carcinogen report on gastric cancer and colorectal cancer released by the U.
    S.
    Department of Health and Human Services
    .

    Image: Diseases caused by Helicobacter pylori infection

    Image: Diseases caused by Helicobacter pylori infection

    Screening methods for Helicobacter pylori mainly include invasive and non-invasive tests
    .
    Invasive tests include endoscopy, which looks for any abnormalities in the upper
    gastrointestinal tract
    .
    Non-invasive tests, including urea breath test, serum antibody test and stool antigen test, are commonly used clinical detection methods and are recommended by many domestic and foreign
    consensuses
    on the treatment of Helicobacter pylori infection .

    Screening methods for Helicobacter pylori mainly include invasive and non-invasive tests
    .
    Invasive tests include endoscopy, which looks for any abnormalities in the upper
    gastrointestinal tract
    .
    Non-invasive tests, including urea breath test, serum antibody test and stool antigen test, are commonly used clinical detection methods and are recommended by many domestic and foreign
    consensuses
    on the treatment of Helicobacter pylori infection .
    Screening Digestive Consensus

    Among them, the urea breath test ( UBT ) is the most commonly used non-invasive method to detect Helicobacter pylori, including 13 urea breath test ( 13 C-UBT ) and carbon 14 urea breath test ( 14 C-UBT ), which The method has the advantages of relatively high detection accuracy and specificity, convenient operation, and unaffected by the patchy distribution of Helicobacter pylori in the stomach.
    However, in the process of use, the detection process still needs to be strictly followed to ensure the accuracy of the detection results.

    .

    Among them, the urea breath test ( UBT ) is the most commonly used non-invasive method to detect Helicobacter pylori, including 13 urea breath test ( 13 13 C-UBT ) and carbon 14 urea breath test ( 14 14 C-UBT ) , this method has the advantages of relatively high detection accuracy and specificity, convenient operation, and is not affected by the patchy distribution of Helicobacter pylori in the stomach.
    accuracy

    .

    So, what are the differences between 13 C-UBT and 14 C-UBT , and how to choose in clinical practice? This article is compiled and summarized to share with fellow students
    .

    So, what are the differences between 13 13 C-UBT and 14 14 C-UBT , and how to choose in clinical practice? This article is compiled and summarized to share with fellow students
    .

    1.
    Principle of urea breath test

    1.
    Principle
    of urea breath test 1.
    Principle of urea breath test

    Generally speaking, there is little bacterial reproduction in the stomach and no urease activity, while Helicobacter pylori is rich in urease, which can decompose endogenous and exogenous urea into NH 3 and CO 2 , and then enter the blood circulation and Excreted from the lungs
    .

    Generally speaking, there is little bacterial reproduction in the stomach and no urease activity, while Helicobacter pylori is rich in urease, which can decompose endogenous and exogenous urea into NH 3 3 and CO 2 2 , and then enter into The blood circulates and is excreted by the lungs
    .

    Therefore, after oral administration of a certain dose of isotope-labeled urea, the presence or absence of Helicobacter pylori infection can be determined by measuring the changes in CO2 labeled with isotope 13 C or 14 C in the exhaled breath .

    Therefore, after oral administration of a certain dose of isotope-labeled urea, the presence or absence of Helicobacter pylori infection can be determined by measuring the change of CO2 labeled with the isotope 13 13 C or 14 14 C in the exhaled breath .

    2.
    The difference between 13 C-UBT and 14 C-UBT

    2.
    The difference between
    13 C-UBT and 14 C-UBT
    2.
    The difference between 13 13 C-UBT and 14 14 C-UBT

    There are currently two tracers for breath testing, 13 C- urea and 14 C- urea
    .
    13 C is a stable isotope with no radiation effect, but the price is higher
    .
    14 C has a longer half-life and a lower price, but has a certain radiation effect
    .

    There are currently two tracers for breath testing, 13 13 C- urea and 14 14 C- urea
    .
    13 13 C is a stable isotope with no radiation effect, but the price is high
    .
    14 14 C has a longer half-life and lower price, but has a certain radiation effect
    .

    13 C-UBT is detected and analyzed by mass spectrometry, and its measured value is collected from two breath samples before and after taking the drug for comparative measurement
    .
    The
    14 C-UBT is measured by liquid scintillation detection method only on the breath samples of the subjects after taking the drug
    .

    13 13 C-UBT was detected and analyzed by mass spectrometry, and its determination value was collected from two breath samples before and after taking the drug for comparative measurement
    .
    The
    14 14 C-UBT was measured by liquid scintillation detection method only on the breath samples of the subjects after taking the drug
    .

    The higher the dose of 13 C-urea, the higher the detection accuracy of 13 C-UBT , but the corresponding cost will increase .
    The average sensitivity and specificity of the 75mg dose are both above 7 % , and its high enough accuracy makes it more clinically recommended by domestic and foreign experts .
    The European Medicines Agency guidelines recommend a dose of 13C - urea in 13C - UBT of 75 mg for adults and 45 mg for children .
    For the dose of 14C-urea in 14C - UBT, the patient only needs to take one capsule of 14C - labeled urea and then blow air into the gas collecting device.
    The capsule dose is
    27.
    8kBq
    ( 0.
    75μCi
    ) / capsule .




    The higher the dosage of 13 13 C-urea, the higher the detection accuracy of 13 13 C-UBT , but the corresponding cost will increase .
    The average sensitivity and specificity of the 75mg dose are both above 7 % , and its high enough accuracy makes it more clinically recommended by domestic and foreign experts .
    The European Medicines Agency guidelines recommend that for the dose of 13 13 C-urea in guideline 13 13 C-UBT , the dose of 13 13 C-urea is 75 mg for adults and 45 mg for children .
    For the dose of 14 14 C- urea in 14 14 C -UBT , the patient only needs to take one tablet of 14 14 C orally .



    After marking the urea capsules, blow air to the gas collecting device, and the capsule dose is 27.
    8kBq
    ( 0.
    75μCi
    ) / capsule
    .

    3.
    Indications for 13 C-UBT and 14 C-UBT

    3, 13 C-UBT and 14 C-UBT indications 3, 13 13 C-UBT and 14 14 C-UBT indications

    The scope of clinical application of 13 C-UBT includes re-examination after anti-Helicobacter pylori treatment ; those with a history of peptic ulcer but unknown Helicobacter pylori status and those under 45 years old with dyspepsia
    .

    The scope of clinical application of 13 13 C-UBT includes re-examination after anti-Helicobacter pylori treatment ; patients with a history of peptic ulcer digestion , but the status of Helicobacter pylori is unknown, and those under 45 years old who have dyspepsia
    .

    14 C-UBT is suitable for: 1 ) indigestion initial diagnosis and follow-up ; 2 ) gastroduodenal ulcer, chronic active gastritis, antral gastritis ; 3 ) lymphoma associated with lymphoid tissue, etc.
    requiring eradication of Helicobacter pylori
    4 ) Patients with gastric cancer prevention or family history of gastric cancer ; 5 ) Patients with gastric discomfort during gastroscopy ; 6 ) Patients with gastric discomfort who are unwilling to undergo gastroscopy ; 7 ) Long-term use of non-steroidal anti-inflammatory drugs drug person
    .

    14 14 C-UBT is suitable for: 1 ) indigestion initial diagnosis and follow-up ; 2 ) gastroduodenal ulcer, chronic active gastritis, antral gastritis ; 3 ) lymphoma associated with lymphoid tissue, etc.
    requiring eradication of helicobacter pylorus
    4 ) Prevention of gastric cancer or those with a family history of gastric cancer ; 5 ) Patients with stomach discomfort during gastroscopy ; 6 ) Patients who are unwilling to undergo gastroscopy but have stomach discomfort ; 7 ) Long-term use of non-steroidal body anti-inflammatory drugs .

    Therefore, to detect Helicobacter pylori, whether to choose 13 C-UBT or 14 C-UBT , the doctor can choose according to the patient's situation
    .
    There is little difference between the two urea breath tests, except in pregnant women, lactating women, and children

    .

    Therefore, to detect Helicobacter pylori, whether to choose 13 13 C-UBT or 14 14 C-UBT , doctors can choose according to the patient's situation
    .
    There is little difference between the two urea breath tests, except in pregnant women, lactating women, and children

    .

    4.
    Precautions for urea breath test

    4.
    Precautions for urea breath test 4.
    Precautions for
    urea breath test

    UBT is the detection of Helicobacter pylori and the monitoring of curative effect after radical treatment.
    It
    is widely used in clinical practice
    .
    However, the efficacy of its detection is still affected by many clinical factors, such as drug use, detection equipment and detection reagents, examiner's operation, diet, and disease

    .

    UBT is the detection of Helicobacter pylori and the monitoring of curative effect after radical treatment.
    It
    is widely used in clinical practice
    .
    However, the efficacy of its detection is still affected by many clinical factors, such as drug use, detection equipment and detection reagents, examiner's operation, diet, and disease

    .

    Therefore, these influences should be avoided in the clinical application process, and the examination should be made more accurate as much as possible to improve the accuracy of diagnosis
    and better serve the clinical guidance of treatment .

    Therefore, these influences should be avoided in the clinical application process, and the examination should be made more accurate as much as possible to improve the accuracy of diagnosis
    and better serve the clinical guidance of treatment .
    diagnosis

    References

    References

    1.
    Digestive Disease Branch of Chinese Medical Association , Digestive Endoscopy Branch of Chinese Medical Association .
    Expert advice on quality control of 13C
    urea breath test [J] .
    Chinese Journal of Digestive Endoscopy
    , 2021, 38(3):4.

    1.
    Digestive Disease Branch of Chinese Medical Association , Digestive Endoscopy Branch of Chinese Medical Association .
    Expert advice on quality control of 13C
    urea breath test [J]
    .
    Chinese
    Journal of Digestive Endoscopy , 2021, 38(3):4.
    1.
    Zhonghua
    Gastroenterology Branch of Medical Association , Digestive Endoscopy Branch of Chinese Medical Association .
    Expert advice on quality control of 13C
    urea breath test [J] .
    Chinese Journal of Digestive Endoscopy
    , 2021, 38(3):4.

    2.
    National Clinical Research Center for Digestive Diseases ( Shanghai ), National Alliance of Early Cancer Prevention and Control Centers of Digestive Tract , Helicobacter pylori and Peptic Ulcer Group of Digestive Disease Branch of Chinese Medical Association , etc.
    Helicobacter pylori infection
    in Chinese households Expert consensus on prevention and control and management (2021 )[J].
    Chinese Journal of Digestive Medicine , 2021, 41(4):13.

    2.
    National Clinical Research Center for Digestive Diseases ( Shanghai ), National Alliance of Early Cancer Prevention and Control Centers of Digestive Tract , Helicobacter pylori and Peptic Ulcer Group of Digestive Disease Branch of Chinese Medical Association , etc.
    Helicobacter pylori infection
    in Chinese households Expert consensus on prevention and control and management (2021 )[J].
    Chinese Journal of Digestive Diseases , 2021, 41(4):13.
    2.
    National Clinical Research Center for Digestive Diseases ( Shanghai ), National Alliance of Early Cancer Prevention and Treatment Centers of Digestive Tract , Helicobacter pylori and peptic ulcer group of Chinese Medical Association Gastroenterology Branch , et al .
    Expert consensus management on prevention, control and management of Helicobacter pylori infection in Chinese households (2021 ) [J].
    Chinese Journal of Digestive Medicine , 2021, 41( 4):13.

    3.
    Liu Yi , Zhong Liang , Sun Dayu .
    Diagnosis of Helicobacter pylori infection ——^13C- urea breath test [J] .
    International Journal of
    Digestive Diseases , 2003, 23(002):85-87.

    3.
    Liu Yi , Zhong Liang , Sun Dayu .
    Diagnosis of Helicobacter pylori infection - ^13C- urea breath test [J].
    International Journal of Digestive Diseases , 2003, 23(002):85-87.
    3.
    Liu Yi , Zhong Liang , Sun Dayu .
    Diagnosis of Helicobacter pylori infection ——^13C- urea breath test [J] .
    International Journal of
    Digestive Diseases , 2003, 23(002):85-87.

    4.
    Huang Hao , Cheng Zhongwei.
    Principle and clinical application of 13C breath test [J] .
    Medical and
    Health Equipment , 2003(S1):1.

    4.
    Huang Hao , Cheng Zhongwei.
    Principle and clinical application of 13 C breath test [J] .
    Medical and
    Health Equipment , 2003(S1):1.
    4.
    Huang Hao , Cheng Zhongwei .
    Principle and clinical application of 13 C breath test [J] .
    Medical
    and Health Equipment , 2003(S1):1.

    5.
    Bai Tao , Hou Xiaohua .
    Influencing factors of Helicobacter pylori infection detected by urea breath test [J].
    Journal of
    Clinical Digestive Diseases , 2020, 32(3):6.

    5.
    Bai Tao , Hou Xiaohua .
    Influencing factors of Helicobacter pylori infection detected by urea breath test [J].
    Journal of
    Clinical Digestive Diseases , 2020, 32(3): 6.
    5.
    Bai Tao , Hou Xiaohua .
    Urea breath test to detect pylori Influencing factors of Helicobacter infection [J].
    Journal of
    Clinical Digestive Diseases , 2020, 32(3):6.


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