Hello, Watson and Crick, I’m Hoogsteen-the development of a triple helix-based DNA allosteric device

Since its inception, DNA nanostructures have shown considerable application prospects in many fields such as drug delivery, environmental monitoring, biocomputing, and smart materials due to their excellent codeability

Allosteric regulation is a very common and efficient way of regulation in life activities that has evolved over a long period of time in nature

In this study, the author designed a Z-switch structure with a triple helix as an allosteric site.

Figure 1.

Next, the author applied the allosteric regulation strategy to imitate some natural proteins, successfully realized the co-regulation mode of multiple allosteric regulation ligands, and realized the selective control of different reflection pathways

Figure 2.

This study expands the application of allosteric regulation in nucleic acid nanostructures, establishes a new regulation model, establishes the screening process, and enriches the regulation mechanism and completion methods.

The research results were completed by the Wei Diming Molecular Design Group (MADlab) of the School of Life Sciences, Tsinghua University.

Link to the paper: https://pubs.