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Heterogeneous biosynthesis of plant volatile terpenoids such as rose essential oil |
Science and Technology Daily reporter Chen Xi
Plant volatile terpenes (PVTs) are the main components of flavor/aroma substances in flowers, fruits and vegetables, including the well-known hop oil, sandalwood oil, rose oil and so on
.
At present, there are more than 18,000 kinds of PVT compounds, most of which are monoterpenes and sesquiterpene compounds, which are widely used in the fields of flavor and fragrance, medicine and chemical industry, food processing, agriculture and so on
In order to achieve alternative production rose essential oils and other compounds PVTs, China Kexue Yuan researcher Zhang Xueli Tianjin Institute of Industrial Biotechnology, the research team led by microbial metabolic engineering, Saccharomyces cerevisiae was the first system reform, and on this basis, by way of introduction and rose oil After optimization, a "rose yeast" strain with a yield of 5 g/L rose essential oil in a 5 L fermentor was finally obtained
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Relevant research results were published in the international journal "Green Chemistry", and 4 patents were applied for
Academy of Sciences
Using synthetic biology technology to create an "artificial cell factory" to fermentatively produce rose essential oil and other PVTs is a promising way
.
Taking the fermentation and production of natural rose essential oil as a case, Zhang Xueli’s team first screened in Saccharomyces cerevisiae that the metabolic shutoff effect was significantly better than the functional element of the classical dynamic regulatory promoter ERG1-the ERG7 promoter element in the ergosterol biosynthetic pathway
On this basis, the geraniol-rose alcohol synthesis module and the nerol synthesis module were integrated into the same chassis strain, creating the world’s first "rose yeast" that simultaneously produces the three main monoterpene components of natural rose essential oil.
Strain: Rose-Yeast 1.
0
.
In the shake flask fermentation, the total monoterpene output of this strain reached 120mg/L, and the ratio of the three components in the natural Damascus rose essential oil was close
In further work, the scientific research team expanded the research and development strategy and developed a platform yeast with high-yield monoterpenes and sesquiterpenes, and the yield of target products has been greatly increased
.
This research laid the foundation for the efficient heterologous biosynthesis of plant volatile terpenoids such as rose essential oil