High-Resolution Proteome/Peptidome Analysis of Body Fluids by Capillary Electrophoresis Coupled with MS
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Last Update: 2021-01-03
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Source: Internet
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Author: User
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The analysis of proteins and peptides in biological fluids is becoming more important as they are potential sources of diagnosticbiomarkers of disease. The complexity of body fluids is such that no single technique can both identify and quantify all theconstituents present. It therefore requires pre-concentration of the compounds of interest and hyphenated techniques likecapillary electrophoresis (CE) or high-performance liquid chromatography (HPLC) coupled to a mass spectrometer (MS) to providethe separation, identification, and quantification of the proteins and peptides under investigation. Here we provide protocolsfor sample preparation of various body fluids, namely, urine, plasma, and cerebrospinal fluid, and how to obtain maximum sensitivityand selectivity of the small proteins and peptides under investigation by CE-MS.
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