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Glycosylation research has gained significant attention in several research fields including immunology, protein production,and biomarker discovery. However, complex and time-consuming protocols are often necessary to obtain suitable samples foranalysis. We here describe a short and robust assay to prepare 8-aminopyrene-1,3,6-trisulphonic acid-labeled N-glycans fromserum samples. It only involves the subsequent addition of reagents and incubation in a
PCR
thermocycler. Moreover, this assayallows the detection of these glycans, which are only present in minute amounts in serum, on high-throughput microfluidicsCE platforms including the MCE-202 MultiNA, 2100 Bioanalyzer, and eGene system. Using this clinical glycomics assay, we couldreliably measure GlycoHepatoTest, a panel of biomarkers allowing the follow-up of chronic liver disease patients from theearly stage onward.