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High-Throughput Screening of Small-Molecule Libraries for Inducers of Plant Defense Responses

 Last Update: 2021-01-13
 Source: Internet
 Author: User

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responses

reporter gene assay

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Transgenic Arabidopsis seedlings containing a pathogen-responsive reporter gene allow for convenient high-throughput screening of chemical libraries for compounds that induce plant defense responses. Candidates identified by such screens can be further tested for their ability to protect plants from pathogen-caused diseases. Using Arabidopsis defense signaling mutants, defined regulatory processes that are targeted by a given candidate molecule can be easily narrowed down. Here, we provide a detailed high-throughput screening protocol for library compounds that activate a pathogen-responsive reporter gene in liquid-grown Arabidopsis seedlings.

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