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HLA DR-DQ Genotyping by Capillary Electrophoresis for Risk Assessment for Celiac Disease

 Last Update: 2020-12-21
 Source: Internet
 Author: User

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methylation specific pcr

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The risk for celiac disease (CD) is clearly related to specific HLA D
QA
1 and DQB1 alleles, but HLA �typing is often consideredtoo costly for frequent use.
Here we present a method using sequence-specific primed
PCR
(PCR-SSP) for HLA-DR-DQ genotyping optimized for capillary electrophoresison Applied Biosystems 3130
xl
Genetic Analyzer. Requiring a total of three PCR reactions and a single electrophoretic step, this method reduces the reagentexpenses and technical time for directed HLA typing to distinguish risk alleles for CD, with a sufficient throughput for large-scalescreening projects.

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