How are hydrolysed casein (MH) agar cultures made?

1. Nutritional broth

protein1.0 g

NaCl 0.5 g

water 100 ml

pH 7.0 to 7.2

in < add water to > ", called beef paste, protein and NaCl,aftermelts. Adjust the pH to 7.0 to 7.2. Sub-pack, add cotton plugs, high-pressure steam sterilization is ready. It is commonly used to culture bacteria.

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adds 20 grams of agar to the nutrient broth medium. It is commonly used to culture bacteria.

3. Gravy protein liquid culture

beef 500g

protein 10g

NaCl 5g

pH 7.1 to 1 7.2

Take 500 grams of fresh beef, clean fat, tendons, crush or crush, add water 1000 ml soaked, 15 degrees C for 12 hours or 50 degrees C for half an hour. Use gauze to filter the gravy < a href" > towater loss. Add protein and saltgravy. Heat the gravy, add the soda (sodium carbonate) to red and adjust the pH. Sub-packed, auto-pressure steam sterilized.

the above-mentioned sterilized medium with cotton filter out the condensation ofprotein, made into a liquid medium, if needed to make a solid medium, can be added to every 100 ml of liquid 2 grams of agar, heated dissolved, divided, again high-pressure steam sterilization. It is commonly used to culture bacteria.

4.Gaussi No. 1 culture (starch agar culture)

soluble starch 2 g

K2HPO4 0.05 g

MgSO4.7H2O 0.05 g

KNO3 0.1 g

NaCl 0.05 g

FeSO4 0.001 g

agar 2 g

water 100 ml

pH 7.2 to 7.4

add water to the beaker 95 ml, heat to boil, take soluble soluble powder 2 g, with 5 ml of water into paste, poured into boiling water and uniform. Also take other drugs, one after another added to the beech (after one drug dissolves, then add a second drug). After all the drugs are dissolved, stop heating, fill with dehydration, and adjust the pH to 7.2 to 7.4. After dislocation, the high-pressure steam is sterilized. This culture base is often used to culture line bacteria.

5. Potato sucrose media

potatoes 200g

sucrose 10g

agar 20g

water 1000ml

natural pH

200g potato chips, 1000ml water, boiled for half an hour, filtered with gauze to fill the water loss. Add 10 grams of sucrose and 20 grams of agar to the juice and heat to melt the agar. After dislocation, the high-pressure steam is sterilized. It is commonly used < culture a href"" > yeastbacteria.

6. Malt Juice Medium

will buy malt juice with hops from the brewery, put it in a conical bottle and sterilise it with high-pressure steam. It is commonly used to culture yeast.

7.Tsa's

Nano3 2g

K2HPO4 1g

KCl 0.5g

MgSO4 0.5g

FeSO 4 0.01 g

cane sugar 30 g

agar 15 to 20 g

water 1000 ml

natural pH

sub-pack, add cotton plug, auto-steam sterilization. It is often used to culture mold.

8. Bean sprout juice glucose (or sucrose) medium

soybean sprouts 100g

glucose 50g

agar 15g

water 1000ml

said fresh bean sprouts 100g, put in a beaker, add 1000ml water, boil for 30 minutes, filtered with gauze to fill the water. Add 50g of glucose (or sucrose) and 15g of agar, heat until melted to make up for the loss of water. Sub-pack, add cotton plugs, high-pressure steam sterilization. It is often used to culture mold.

9.Ihong Mei blue culture (E, M, B culture)

Lactose 10 g

protein 5 g

NaCl 5 g

K2HPO4 2 g

2% Yihong aqueous solution 20 ml

0.35% melaninal solution 20 ml

agar 20g

water 1000ml

pH 7.2