How the filler (DEAE-cellulose 52) for layering regenerates and re-interests.

asked: "Please ask, for the layering offiller (DEAE-cellulose 52) how to regenerate, reuse, how to deal with preservation and reuse? I am now experimenting to this point, do not know how to deal with, so anxious, please give some advice! Thank you!

In addition, before my layering withacid-base treatment DEAE-cellulose 52, washing for a long time is not the appropriate pH, acid-base treatment took a day, please the master can tell me the detailed processing process, let me check if I have any problems. Thank you so much!!

take 0.5M NaOH 500 ml (add NaCl to make the concentration of 0.5M NaCl), called DEAE-cellulose DE-52 30g, sprinkled into the above liquid, mixed. Place 2h at room temperature, for 0.5M NaOH once.

DEAE-52 regeneration method:

1. Generally speaking, simply wash with high salt, the conductivity (the inverted resistance) can reach about 1.0, about 0.35M NaCl.

2. Provides a more efficient method for regenerating columns. 0.1M Na3PO4-0.2% Triton X-100 for more complex proteins and more heavily contaminated columns.

The second method I give you should be a relatively strong recycled washing liquid, this method is also I recently checked the literature, because I recently found that high-salt washing is not thorough, so found this method, washing with a large amount of water to remove the surfactant, and then use the upper column start buffer balance.